胶原蛋白在乙酸酯类离子液体中的溶解及再生性能  

Solubility and regeneration characteristics of collagen in acetic ester-containing ionic liquids

作　　者：郭佳荣[1] 王岩[2] 柏韵宁 李森[2] 霍东娟 王国伟[2] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009 [2]南京工业大学食品与轻工学院,江苏南京210009 [3]南京工业大学理学院,江苏南京210009

出　　处：《南京工业大学学报（自然科学版）》2015年第1期20-26,共7页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：江苏省基础研究计划(自然科学基金)(BK2011799);高等学校博士学科点专项科研基金联合资助课题(20113221120006);江苏省高等学校大学生实践创新训练计划(2012JSSPITP3054)

摘　　要：合成了2种乙酸酯类离子液体3-甲基-1-乙氧羰基甲基咪唑氯盐（[Etmim] Cl）、3-甲基-1-乙氧羰基甲基咪唑溴盐（[Etmim] Br）,通过核磁共振氢谱（1H NMR）和元素分析确认目标产物化学结构.比较胶原蛋白分别在2种离子液体中的溶解能力大小,并分别探讨了4种不同再生试剂对胶原蛋白再生情况的影响,如去离子水、甲醇、乙醇、丙酮.对以（[Etmim] Cl）离子液体为溶剂、甲醇为再生剂的再生前后胶原蛋白进行傅里叶变换红外光谱（FT-IR）分析.结果表明：2种离子液体都是胶原蛋白的直接溶剂,且（[Etmim] Cl）对胶原蛋白的溶解时间比1-烯丙基-3-甲基咪唑氯盐[AMIM] Cl和1-丁基-3-甲基咪唑氯盐[BMIM] Cl缩短了15％～20％,在140℃时,[Etmim] Cl溶解固体质量分数为8％的胶原蛋白仅需15 min,同时胶原蛋白再生前后结构并未发生变化.Two kinds of acetic ester-containing ionic liquids, 3-methyl-l-（ethoxycarbonylmethyl） imidazolium chloride （ [ Etmim] Cl ） and 3-methyl-l-（ ethoxycarbonylmethyl ） imidazolium bromide （[ Etmim] Br） were synthesized and characterized by 1H nuclear magnetic resonance hydrogen spectrum （1H NMR） and elemental analysis. The solubility of collagen in the ionic liquids was investigated. Different solvents as regenerant for regeneration of collagen, such as deionized water, methanol, ethanol, and acetone were discussed. The dissolution and regeneration mechanism of collagen was studied by Fourier transform infrared spectroscopy （FT-IR） which was dissolved by [ Etmim] Cl and regenerated by methanol. Results showed that both ionic liquids were good solvent to collagen, and the dissolution time of [Etmim] C1 to collagen was about 15%-20% shorter than that of [ AMIM] Cl and [ BMIM] Cl. When the temperature was 140 ℃, the collagen content was 8% ,the required time of [Etmim] Cl was just about 15 min. Also, there were no difference between raw collagen and regenerated collagen.

关 键 词：胶原蛋白 乙酸酯类离子液体 溶解 再生 

分 类 号：O621.3[理学—有机化学]