黑曲霉(Aspergillus niger)发酵生产木聚糖酶的pH调控策略  

Two-stage pH control strategy for xylanase production by Aspergillus niger D08

作　　者：邢亚梅 陈勇[1] 应汉杰[1] 

机构地区：[1]南京工业大学生物与制药工程学院国家生化工程技术研究中心,江苏南京211800

出　　处：《生物加工过程》2015年第1期23-27,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家杰出青年基金(21025625);国家高技术研究发展计划(863计划)(2012AA021203);国家科技支撑计划(2012BAI44G01);国家自然科学基金面上基金(21376118);国家自然科学基金青年基金(21106070);长江学者和创新发展计划(PCSIRT);江苏自然科学基金(SBK20150207);江苏省高校优势学科建设工程项目(PAPD)

摘　　要：为了提高黑曲霉(Aspergillus niger)D08生产木聚糖酶的能力,考察了不同p H条件对菌株生长及产酶的影响,结果发现木聚糖酶发酵过程中菌体生长的最适p H与木聚糖酶合成的最适p H不同,分别为3.5和4.5。由此针对性地提出了两阶段p H控制策略:在30 h内控制p H为3.5;30 h后p H控制为4.5。结果表明:分阶段p H调控策略的实施进一步提高了菌体合成木聚糖酶的能力,木聚糖酶比酶活(2 223.38 U/m L)和生产强度(26.47U/(m L·h))分别比恒定p H 4.5时提高了14.3%和30.6%。该工艺对提高木聚糖酶活力具有一定的指导意义,为后续研究和生产奠定了良好的基础。In order to improve xylanase production of Aspergillus niger D08, effects of pH on cell growth and xylanase production were investigated. The optimum pH for cell growth was 3.5, while that for xylanase production was 4.5. Accordingly, a two-stage pH control strategy was as follows： pH was controlled at 3.5 for the first 30 h, and then raised to 4.5 for the remaining time. With this strategy, the maximal xylanase activity and productivity of xylanase reached 2 223.38 U/mL and 26.47 U/（mL·h） , respectively, which were 14.3% and 30.6% higher than those in the single pH （pH 4.5） controlled model. This pH control strategy offers a promising guideline for enhancing the production of xylanase by Aspergillus niger D08, thus making a good foundation to further research and production of xylanase.

关 键 词：pH调控 木聚糖酶 黑曲霉 

分 类 号：Q815[生物学—生物工程]