白香丹胶囊对经前期综合征肝气逆证模型大鼠学习记忆功能的影响  

Effects of Baixiangdan capsule on learning and memory in PMS rat model with liver-qi invasion syndrome

作　　者：宗绍波[1] 朱德豪 魏盛[2,3] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室、江苏省企业院士工作站,江苏连云港222001 [2]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355 [3]山东中医药大学脑行为分析实验室,山东济南250355

出　　处：《中国药理学通报》2015年第2期284-289,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年基金资助项目(No 81202617)

摘　　要：目的以经前期综合征(premenstrual syndrome,PMS)肝气逆证大鼠模型为载体考察白香丹胶囊对模型大鼠学习记忆功能的影响,探讨行为学表现下病证对学习记忆功能的损伤及白香丹胶囊的干预效应。方法应用情志刺激为主,多因素分段刺激法制备PMS肝气逆证大鼠模型并进行药物干预,采用旷场实验评价模型。采用Morris水迷宫、Y迷宫、新物体识别实验对PMS肝气逆证模型大鼠空间学习记忆能力和非空间学习记忆能力进行检测。结果与正常组大鼠相比,模型组大鼠总路程明显增加,进入中央区次数及中央区停留时间均明显降低;与模型组相比,白香丹组大鼠总路程明显下降,中央区停留时间及进入中央区次数均明显升高,氟西汀组大鼠总路程明显下降。与正常组相比,模型组大鼠分辨指数(distinguish index,DI)明显下降,白香丹组大鼠DI明显升高;定位航行阶段,与正常组大鼠相比,模型组大鼠在d 2、4逃避潜伏期明显上升;与模型组相比,白香丹和氟西汀组大鼠在d 2、4的逃避潜伏期明显降低;空间探索阶段,与正常组相比,模型组大鼠逃避潜伏期明显上升,而平台穿越次数明显减少,与模型组相比,白香丹组大鼠平台穿越次数明显增多。结论 PMS肝气逆证模型大鼠伴有学习记忆功能损伤,白香丹胶囊能够纠正模型大鼠的学习记忆功能缺陷,且治疗效果可能优于氟西汀。Aim To explore the effects of Baixiangdancapsule on learning and memory in PMS rat model withliver-qi invasion syndrome. Methods Liver-qi invasion rat model was prepared using foot-shock and noise stimulation, and then the model was treated with drugs,finally all the group of rats were evaluated using open-field test. We also detected learning and memory function of PMS liver-qi invasion rats model using Ymaze,new-object recognition and Morris water maze test. Results Compared with the normal group,the total distance increased significantly,number of crosses into the central area and duration of movement in the central area decreased significantly in model group.The total distance decreased significantly,number of crosses into the central area and duration of movement in the central area increased significantly in Baixiangdan treatment group compared to the model group,and the total distance decreased significantly in fluoxetine treatment group compared to the model group. Compared with the normal group,DI decreased significantly in model group,and DI increased significantly inBaixiangdan treatment group. During place navigation training,the model group's escape latency was obviously delayed on 2nd,4th day. Compared with the model group, Baixiangdan and fluoxetine treatment group's escape latency was obviously reduced. During spatial probe test,compared with the normal group,the escape latency was obviously delayed, and the number of crossing platforms was significantly reduced in the model group. Compared with the model group,the number of crossing platforms significantly increased in Baixiangdan treatment group. Conclusion PMS liver-qi invasion rat model presents learning and memory damage,which could be improved by Baixiangdan capsules,and the treatment effect may be superior to that of fluoxetine.

关 键 词：白香丹胶囊 经前期综合征 肝气逆证 大鼠模型 学习记忆 Morris水迷宫 旷场实验 

分 类 号：R-332[医药卫生] R285.5