HPLC法快速测定熟肉制品中10种食品添加剂  

Determination of 10 Food Additives in Meat Products by High Performance Liquid Chromatography

作　　者：王静静[1] 巩志国[1] 苏敏[1] 李世雨[1] 尚德军[1] 

机构地区：[1]新疆出入境检验检疫局检验检疫技术中心,乌鲁木齐830063

出　　处：《化学分析计量》2015年第1期64-67,共4页Chemical Analysis And Meterage

摘　　要：建立了熟肉制品中10 种食品添加剂的高效液相色谱分析方法.以乙醇– 氨水溶液为提取溶剂,样品经超声波辅助溶剂萃取法萃取后,以Atlantis d C18（250 mm×4.6 mm,5 μm） 色谱柱为分离柱,20 mmol/L 乙酸铵– 甲醇为流动相进行分离测定.10 种目标化合物的质量浓度在1.0～200.0 mg/L 范围内与其峰面积线性关系良好（r 〉0.999）,在20.0,500.0,1 000.0 mg/kg 的添加水平下,10 种目标化合物的平均加标回收率为82%～104%,测定结果的相对标准偏差小于4.0% （n=6）,方法的检出限为1.0～5.0 mg/kg （S/N=3）.该方法简单、快速、准确,适合于熟肉制品中食品添加剂的检测.A comprehensive analytical method based on high performance liquid chromatography（HPLC） wasdeveloped for determining 10 food additives in meat products. Samples were extracted by ethanol–ammonia solutionwith ultrasonic wave assistant extraction（UAE） and then determined. The Atlantis d C18 column（4.6 mm×250 mm,5.0μm） was used to complete the separation of the analyst with 20 mmol/L ammonium acetate–methanol as mobile phase.The mass concentration of 10 food additives had linear relationship with the peak area in the range of 1.0–200.0 mg/L,the correlation coefficients（r） were more than 0.999. Recoveries for 10 food additives ranged from 82% to 104% at thespiked level of 20.0,500.0, 1 000.0 mg/kg and the relative standard deviations of determination results were less than4.0% （n=6）. The detection limits of 10 food additives ranged from 1.0 mg/kg to 5.0 mg/kg. The results indicate thatthe method is easier,faster,safty,and suitable for the qualitative and quantitative confirmation of the 10 food additivesin meat products.

关 键 词：高效液相色谱法 熟肉制品 食品添加剂 

分 类 号：O657.7[理学—分析化学]