促进氧化溴酚蓝光度法测定饲料中牛磺酸含量  

Spectrophotometric determination of taurinr content in feed by promoting bromophenol blue oxidation

作　　者：李人宇[1,2] 彭松[1] 李咏梅[2] 陶明涛[1] 

机构地区：[1]连云港师范高等专科学校,江苏连云港222006 [2]江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《中国饲料》2015年第2期31-33,37,共4页China Feed

基　　金：2014年江苏省高校"青蓝工程"中青年学术带头人项目;江苏省海洋资源开发研究院开放课题基金项目(JSIMR201308);2014年江苏省高等学校大学生创新创业训练计划项目(233);淮海工学院江苏省海洋生物技术重点实验室研究基金项目(2012HS007)

摘　　要：本实验研究了牛磺酸对高锰酸钾氧化溴酚蓝反应的促进作用，建立了分光光度法测定牛磺酸的新方法。结果表明：最大吸收波长为400nm，牛磺酸浓度为0～0．15μg／mL时符合比耳定律，表观摩尔吸光系数为6．25x10^5L／mol·cm，检出限为2．63μg／L。方法用于测定饲料中牛磺酸含量，结果与高效液相色谱法一致，标准偏差为O．85％～1．31％（n=6），回收率为97．0％～101．8％。A new spectrophotometric method for determination of taurine was developed after the promotive effect of taurine on the reaction of potassium hypermanganate oxidizing bromophenol blue was studied.The results showed that the maximum absorption wavelength located at 400 nm.The Beer＇s law was obeyed in the range of 0 - 0.15 μg/mL for taurine with the apparent molar absorption coefficient of 6.25x10^5 L/（mol-cm）and the detection limit of 2.63 μg/L.This method was applied to the determination of taurine content in feed.The results agreed well with those obtained by high performance liquid chromatography.The relative standard deviation was 0.85% x 1.31% （n=6）,and the average recovery was 97.0%- 101.8%.

关 键 词：分光光度法 牛磺酸 溴酚蓝 饲料 测定 

分 类 号：S816.17[农业科学—饲料科学]