酵母菌0732-1产生的抑细菌物质特性研究  

Characteristics of antibacterial substance produced by the yeast strain 0732-1

作　　者：王晓东 王春娟 毛晓英 张建 李国庆[2] 

机构地区：[1]石河子大学绿洲农作物病害防控重点实验室,石河子大学农学院,石河子832000 [2]农业微生物国家重点实验室,华中农业大学,武汉430070

出　　处：《植物病理学报》2015年第1期106-109,共4页Acta Phytopathologica Sinica

基　　金：国家青年科学基金项目(31000874);国家公益性行业(农业)专项(201003066);石河子大学高层次人才科研启动项目(RCZX200906)

摘　　要：哈密瓜细菌性果斑病是近年来暴发流行的一种瓜类病害，也是我国对内对外检疫性重大植物病害。该病由燕麦嗜酸菌西瓜亚种（Acidovorax avenae subsp．citrulli，Aac）引起，具有发病快、危害广、损失重、防治难的特点，一旦发生往往给瓜产区造成严重经济损失。Bioactive metabolites produced by Pichia anomala 0732-1 displayed significant antibacterial activity to Acidovorax avenae subsp, citrulli （Aac） which caused bacterial fruit blotch. The antibiotic capacity, antibac- terial activity and stability of crude antagonistic extract of P. anomala 0732-1 were tested and the active sub- stances were isolated. The results showed that the antibacterial material was not sensitive to ultraviolet radiation and temperature, and it was stable at pH 〈 9. The antibacterial material was purified by precipitation with （NH4）2SO4 （10%-100%, w/v）, extraction with benzene, acetone, chloroform, phenixin, ethanol and ethyl acetate, and distillation method. However, it could not be purified by precipitation with （NH4 ）2 SO4 or by extraction with organic solvent. It could be partially distilled out by distillation. In addition, antibacterial substances produced by P. anomala 0732-1 had significant inhibitory effect against Hami melon seed infested by Aac （bacterial suspension 1×10^8 CFU/mL）. When infected seeds were treated with crude antagonistic extract of P. anomala 0732-1 for 120 min, the seeding disease index was 1.67 during the cotyledon. This study suggested that P. anomala 0732-1 had the potential to be developed as an antagonistic agent for control Hami bacterial fruit blotch.

关 键 词：细菌性果斑病 酵母菌 特性 物质 AVENAE 瓜类病害 暴发流行 植物病害 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]