瘤胃纤毛虫的壳多糖酶  

Chitinase enzymes of the rumen ciliate

作　　者：隋洪楠 

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《天津化工》2015年第1期29-31,共3页Tianjin Chemical Industry

摘　　要：瘤胃中的纤毛虫消化角素的能力是广为人知的。我们研究了瘤胃中的纤毛虫的壳多糖分解系统。这些纤毛虫在特定的动物群体内生长。它们从瘤胃中分离出来,然后通过离心纯化获得。用抗生素孵化纤毛虫后,再准备好粗酶。这样做是为了防止它们被细胞内的细菌污染。特定酶的活性是根据释放的物质的数量来确定的。有研究表明,p H4.5~5.5,温度45~55℃是获得所测活性的最佳条件。研究发现β-N-乙酰基葡萄糖苷酶具有最高的活性（每小时每毫克蛋白质,释放4.2μmol/L产物）。内壳多糖酶和外壳多糖酶的活性不到β-N-乙酰基葡萄糖苷酶的一半。酶谱研究表明在实验制剂中出现了两种内壳多糖酶,两种外壳多糖酶和两种β-N-乙酰基葡萄糖苷酶。The ability of rumen ciliates to digest chitin is clearly recognized. We investigated the chitinolytic system of the rumen ciliate Eudiplodinium maggii. The ciliates were grown in a selectively faunated. They were isolated from the rumen and purified by sedimentation. A crude enzyme preparation was prepared following incubation of ciliates with antibiotics. This was done in order to reduce their contamination with intracellular bacteria. The activity of particular enzymes was examined by quantification of the products released from specific substrates. It was stated that the optimum conditions for the detected activities varied between 4.5 and 5.5 pH, and 45 and 55 °C. β-N- Acetyl-glucosaminidase was found as an enzyme of the highest activity（4.2 μmol/l released product per mg protein per h）. The activities of endochitinase and exochitinase were almost two times lower than that of β- N- acetylglucosaminidase. Zymographic studies revealed the presence of two endochitinases, two exochitinases and two β-N-acetylglucosamini- dases in the examined preparation.

关 键 词：瘤胃纤毛虫 壳多糖酶 降解 聚丙烯酰胺电泳 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]