PIAS3过表达对肺腺癌细胞A549增殖的影响及意义  

Effect of PIAS3 over expression on proliferation in adenocarcinoma A549 cells and the significance

作　　者：俞庆华[1] 李峰生 黄乃祥[1] 高玲[3] 

机构地区：[1]解放军第307医院胸外科,北京100071 [2]第二炮兵总医院全军核辐射损伤医学监测与防治研究中心,北京100088 [3]中国疾病预防控制中心辐射防护与核医学安全所辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《中国医学装备》2015年第1期10-13,共4页China Medical Equipment

基　　金：国家自然科学基金(31340051)"腺病毒Ad-pig3RRP-PIAS3对辐射诱导肺腺癌细胞转移的抑制作用及其机制研究";国家自然科学基金(81202151)"低剂量辐射对树突状细胞迁移能力的影响及其分子机制研究";国家自然科学基金(81001216)"NOK激酶促进肿瘤细胞周期进程及提高肿瘤细胞辐射敏感性分子机制研究";全军医学科技"十二五"科研项目(CWS12J082)"快速PCC-FISH方法作为辐射生物剂量计的研究"

摘　　要：目的:探索活化型信号转导子和转录激活子3蛋白抑制剂(PIAS3)对肺腺癌A549细胞增殖的影响及相关意义,为进一步研究利用辐射应答基因启动子驱动的PIAS3腺相关病毒载体联合放射治疗肺腺癌提供基础。方法:利用MTT比色试验和克隆形成率实验检测转染p CMV-Sport6-PIAS3后对肺腺癌细胞A549存活和增殖的影响;利用免疫荧光染色实验检测共转染p CMVSport6-PIAS3、PRC-CMV、PRC-STAT3及PRC-STAT3-CT后对p-STAT3入核的影响。结果:PIAS3过表达导致A549细胞生长显著抑制,克隆形成能力显著降低;免疫荧光染色实验结果表明,转染p CMV-Sport6-PIAS3后,STAT3入核被显著抑制,通过共转染表达STAT3和STAT3-CT后,STAT3入核仍显著受到抑制。结论:PIAS3过表达可抑制STAT3的入核,继而阻断其功能,从而降低肺腺癌细胞A549的生长和增殖能力。Objective： To explore the effect of PIAS3 on proliferation of lung adenocarcinoma A549 cells, in order to provide a foundation for the further study in which a adeno-associated virus （AAV） containing PIAS3 gene driven by a radiation response promoter plus radiotherapy will be used to treatment lung adenocarcinoma. Methods： The growth and proliferation of A549 cells transfected with pCMV-Sport6-PIAS3 plasmid were detected by MTT assay and cloning forming efficiency assay. The nucleus translocation of p-STAT3 was detected by immunofluorescence staining after CMV-Sport6-PIAS3 was co-transfected with PRC-CMV, PRC-STAT3 or PRC-STAT3-CT. Results：The growth and cloning form of A549 cells were significantly inhibited by PIAS3 overexpression. The result of immunofluorescence staining indicated that the nucleus translocation of p-STAT3 as significantly blocked by transfection with pCMV-Sport6-PIAS3, even after co-transfection with STAT3 or STAT3-CT. Conclusion： The overexpression of PIAS3 could inhibit the nucleus translocation of STAT3, which subsequently blocked the function of STAT3 and decreased the ability of growth and proliferation of A549 cells.

关 键 词：活化型STAT3抑制蛋白 肺腺癌 细胞存活 细胞增殖 磷酸化信号转导子和转录激活子3 

分 类 号：R734.2[医药卫生—肿瘤]