细菌σ^(54)启动子序列分析与预测  

The Sequence Analysis and Prediction of σ^(54) Promoter in Bacteria

作　　者：丁辉[1] 邓恩泽 陈伟[2] 林昊[1] 

机构地区：[1]电子科技大学生物信息学中心,成都610054 [2]河北联合大学基因组学与计算生物学中心,河北唐山063000

出　　处：《电子科技大学学报》2015年第1期147-149,共3页Journal of University of Electronic Science and Technology of China

基　　金：国家自然科学基金(61202256;61301260;61100092);中央高校基本科研业务费(ZYGX2012J113;ZYGX2013J102)

摘　　要：对实验确定的168条σ54启动子序列进行保守性分析,获得两个保守的区域-24区域和-12区域,均为最保守的功能元件。选取保守性最大的17个保守位点的三联体频数作为参数,引入伪计数构建位置权重矩阵,对168条σ54启动子进行预测,分别从编码区和汇聚非编码区共选取168条序列组成阴性集。使用Jackknife交叉验证法对模型进行检验,整体准确度达到82.0%,为σ54启动子的理论和实验研究提供新信息。By analyzing the 168 experimental-confirmed σ54 promoter sequences, two conservative regions that are -24 and-12 regions are obtained. The trimer frequency at 17 positions in these conservative regions is selected as inputting parameter. By adding pseudo-count into position weight matrix, the σ54 promoter can be predicted. The 168 negative sequences are extracted from coding regions and convergent intergenic regions. In Jackknife cross-validation, the overall accuracy reaches to 82.0%, suggesting that the model can be further used in the theoretical and experimental study ofσ54 promoter.

关 键 词：细菌 保守性 位置权重矩阵 启动子 

分 类 号：Q61[生物学—生物物理学]