肝癌中NDRG2、AKT2 mRNA及其磷酸化蛋白的表达  

Expressions of NDRG2,AKT2 mRNA and Their Phosphorylated Proteins in Human Hepatic Carcinoma Cells

作　　者：吴国强[1] 秦海明[2] 王大全[1] 

机构地区：[1]沈阳军区总医院普通外科,沈阳110840 [2]沈阳军区总医院病理科,沈阳110840

出　　处：《解放军医药杂志》2015年第1期73-77,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：辽宁省自然科学基金(201204747)

摘　　要：目的检测不同病理级别原发性肝细胞癌中N-myc下游调节基因2(NDRG2)和蛋白激酶B2(AKT2)mRNA及其磷酸化蛋白的表达水平。方法收集不同病理级别原发性肝细胞癌组织及癌旁正常肝组织标本各30例,检测标本中NDRG2、AKT2 mRNA及其磷酸化蛋白的表达情况,并对其间的相互关系进行分析。结果 30例原发性肝细胞癌组织中NDRG2 mRNA表达低于瘤旁组织(P<0.05),AKT2 mRNA与瘤旁组织无明显差异(P>0.05),两者均与病理级别无关(P>0.05),且NDRG2与AKT2表达无相关性(P>0.05)。在高表达474位丝氨酸磷酸化的AKT2蛋白(P-AKT2-SER474)分子的标本中,两个NDRG2磷酸化分子多为低表达,相反,在低表达P-AKT2-SER474分子的标本中,两个NDRG2磷酸化分子多为高表达。结论在mRNA表达水平,还不能认为NDRG2与AKT2之间有相关性。在蛋白质水平,NDRG2的磷酸化不依赖于AKT2的磷酸化,且NDRG2与AKT2的磷酸化作用可能存在竞争关系。Objective To investigate the expressions of N-myc downstream regulated gene 2（ NDRG2）,protein kinase B2（ AKT2） mRNA and their phosphorylated proteins in human hepatic carcinoma cells（ HHCC） of different pathological grades. Methods The expressions of NDRG2,AKT2 mRNA and their phosphorylated proteins in 30 cases of HHCCs（ group A） and 30 cases of adjacent normal tissues（ group B） were detected,and the correlations were also analyzed. Results The NDRG2 mRNA expression in group A was significantly lower than that in group B（ P〈0. 05）,but there was no significant difference in AKT2 expression between the two groups（ P〉0. 05）,and expressions of NDRG2 mRNA and AKT2 were not correlated with pathological grades,furthermore there was no statistically significant correlation between NDRG2 mRNA and AKT2 mRNA expressions（ P〉0. 05）. The expressions of two kinds of phosphorylated NDRG2 proteins were low in the samples in which the phosphorylated AKT2 protein serine474（ P-AKT2-SER474） was highly expressed,and the expressions of two kinds of phosphorylated NDRG2 proteins were high in the samples where the P-AKT2-SER474 was slightly expressed. Conclusion The mRNA expression can not show the correlation between NDRG2 and AKT2. The protein levels show that the NDRG2 phosphorylation does not depend on the AKT2 phosphorylation,and competitive mechanisms may exist in the two processes.

关 键 词：NDRG2 原癌基因蛋白质c-akt 癌 肝细胞 实时聚合酶链反应 印迹法 蛋白质 

分 类 号：R730.261[医药卫生—肿瘤] R392-33[医药卫生—临床医学]