手性修饰氧化石墨衍生物对小鼠成神经瘤细胞活性的影响  

Influence of chirally modified graphite oxide derivatives on activity of mouse neuroblastoma cells N2a

作　　者：杨景霏 廖进齐 陈敏[2] 蔡小康[2] 李仲娟[2] 

机构地区：[1]湖北省武钢三中,湖北武汉430080 [2]湖北理工学院,湖北黄石435003

出　　处：《检验医学与临床》2015年第2期153-154,共2页Laboratory Medicine and Clinic

基　　金：教育部留学生启动资金(46-1);湖北理工学院创新团队项目组(201025)

摘　　要：目的比较手性修饰的氧化石墨（GO）衍生物对小鼠成神经瘤细胞N2a活性的影响。方法倒置荧光显微镜观察N2a细胞形态,MTT法检测N2a细胞的活性。结果在0.1-0.4μmol/L浓度GO衍生物对N2a细胞活性影响呈浓度相关,其中0.4μmol/L的GO衍生物对N2a细胞生长状况最好;L-型GO（L-GO）和D-型GO（D-GO）促N2a细胞增殖率分别为94%和52%,二者差异有统计学意义（P〈0.01）。结论手性修饰的GO衍生物在0.1-0.4μmol/L能促进N2a细胞生长,L-GO促进N2a细胞生长活性比D-GO强。Objective To compare the influence of chirally modified graphite oxide （GO） derivatives on the ac‐tivity of mouse neuroblastoma N2a cells .Methods The morphological change of N2a cells was observed by the inver‐sion fluorescence microscope and the activity of N2a cells was measured by the MTT method .Results The influence of GO derivatives in the concentration range of 0 .1-0 .4 μmol/L on the activity of N2a cells showed the concentra‐tion correlation ,in which the GO derivatives with the concentration of 0 .4μmol/L was best for the growth status of the N2a cells ;the promoting N2a cells proliferation rates of the L‐type GO and D‐type GO were 94% and 52% re‐spectively ,the difference between them revealed the statistical significance（P〈0 .01） .Conclusion The chirally modi‐fied GO derivatives in the concentration range of 0 .1-0 .4μmol/L could promote N2a cell growth ,and the activity of L‐GO promoting N2a cell growth is stronger than that of D‐GO .

关 键 词：氧化石墨 小鼠成神经瘤细胞 细胞活性 

分 类 号：R749.160.2[医药卫生—神经病学与精神病学]