Ebp1的表达对乳腺癌细胞增殖和侵袭的影响  

Influence of the expression of Ebp1 on the proliferation and invasion of breast cancer cells

作　　者：刘媛[1] 袁杰[1] 张洁[1] 赵元元[1] 牛瑞芳[1] 

机构地区：[1]天津医科大学肿瘤医院肿瘤研究所,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,天津300060

出　　处：《天津医科大学学报》2015年第1期14-17,共4页Journal of Tianjin Medical University

摘　　要：目的:研究降低Ebp1的基因表达水平对乳腺癌细胞T47D野生型增殖能力和侵袭能力的影响。方法:转染并筛选Ebp1降表达的单克隆细胞株clone1、clone2、clone3,阴性对照组命名为control。应用小干扰RNA(si RNA)技术降低Ebp1的表达,采用Western blotting技术检测Ebp1蛋白水平的变化,噻唑蓝(MTT)比色法和克隆形成实验观察其对T47D野生型细胞增殖能力和克隆形成能力的影响;采用Matrigel侵袭实验研究其对细胞侵袭能力的影响。结果:Western blotting检测表明si RNA干扰后T47D野生型细胞中Ebp1的蛋白表达水平明显下降,且与亲本和control组细胞比较,Ebp1降表达的细胞克隆的增殖能力和侵袭能力明显增强,差异有统计学意义(P<0.05)。结论:Ebp1表达下降可以明显促进乳腺癌细胞的增殖和侵袭能力,提示Ebp1可能是乳腺癌增殖和侵袭的抑制性因子。Objective： To study the influence of the expression of Ebp 1 on the proliferation and invasion of breast cancer cells. Methods： Western blotting and siRNA were applied to inspect and down-regulate the expression of Ebpl. The effect of proliferation and invasion of T47D wild type cells was examined by MTT assay, colony formation assay and transwell assay respectively. Results： The proliferation and invasion of T47D wild type could be promoted by knock-down of Ebpl as compared to wild type group and control group （P〈0.05）. Conclusion： Down-regulation of Ebpl facilitates the proliferation and invasion of breast cancer cells which suggest that Ebpl may be an inhibiting factor for breast cancer.

关 键 词：EBP1 乳腺癌 增殖 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]