氨肽酶N在乳腺癌血管生成中的作用及其与VEGF的相关性  

Functions of aminopeptidase N in breast cancer angiogenesis and its correlation with VEGF

作　　者：曹文庆[1] 宁殿宾[1] 冯丽[1] 赵玉哲[1] 张静[1] 

机构地区：[1]河北省人民医院普外一科,石家庄050051

出　　处：《天津医科大学学报》2015年第1期39-42,共4页Journal of Tianjin Medical University

摘　　要：目的:研究氨肽酶N(APN)在乳腺癌的表达及临床意义,比较其与血管内皮生长因子(VEGF)的关系,探讨APN在乳腺癌血管生成中的作用。方法:采用免疫组织化学SP法检测APN在60例乳腺癌组织、20例癌旁正常乳腺组织中的表达。结果:APN主要表达于肿瘤细胞的细胞膜,APN在乳腺癌上的表达率[36.7%(22/60)]明显高于癌旁正常乳腺组织[0%(0/20)](χ2=10.115,P=0.001);APN在乳腺癌上的表达与病理类型有关(P=0.035)。乳腺癌组织内还可见新生血管内皮着色,而癌旁正常组织血管内皮无着色;相关性分析显示,乳腺癌组织APN与VEGF的表达无直线相关关系(rs=0.078,P=0.553),但VEGF阳性组APN表达率[44.7%(21/47)]高于VEGF阴性组[7.7%(1/13)](χ2=4.512,P=0.034)。结论:APN与VEGF在乳腺癌血管发生中可能存在一定的协同作用,乳腺癌中APN的表达可提示预后不良,APN可以作为抗肿瘤血管药物中有力的靶点。Objective： To investigate the expression and significance of aminopeptidase N （APN） in breast cancer, meanwhile, to explore the relationship between APN and vascular endothelial growth factor （VEG1O, and to discuss the functions of APN in angiogenesis of breast cancer. Methods： Immunohistochemistry （SP means） was performed to measure the expression of APN in 60 cases of breast cancer tissues and 20 cases of adjacent normal breast tissues. Results： APN mainly was expressed in the membrane of malignant cells, and the positive rate of APN expression in breast cancer tissues was higher than that in adjacent normal breast tissues, 36.7% （22 out of 60） and 0% （0 out of 20） respectively （x^2=10.115, P=0.001）. APN expression was associated with tumor pathological type （P=0.035）. Furthermore, APN expression in neovascular endothelium was also observed in breast cancer tissues while no expression was found in normal endothelium. Under the correlation analysis, no linear correlation was found between the APN expression and VEGF expression （rs=0.078, P=0.553）. However, the APN positive rates in VEGF positive group and in VEGF negative group were 44.7%（21 out of 47）, 7.7% （1 out of 13） respectively, showing the APN expression was more often observed in VEGF positive group than in VEGF negative group （x^2=4.512, P=0.034）. Conclusion： A synergistic effect may exist between APN and VEGF in the function of tumor angiogenesis in breast cancer, and the expression of APN in breast cancer may indicates a poor prognosis. APN could function as an effective target in anti-cancer therapy.

关 键 词：氨肽酶N/CD13 免疫组织化学 乳腺肿瘤 血管内皮生长因子 

分 类 号：R737.9[医药卫生—肿瘤]