莫诺苷对大鼠脑缺血再灌注皮层Wnt信号通路转录因子表达的影响  

Effects of Morroniside on Wnt Signaling-related Transcription Factors in Ischemic Ipsilateral Cortex of Rats after Cerebral Ischemia-reperfusion

作　　者：艾厚喜[1] 孙芳玲[1] 侯虹丽[1,2] 张丽[1] 王文[1] 

机构地区：[1]首都医科大学宣武医院动物实验室,北京市老年病医疗研究中心,北京脑重大疾病研究院,北京市100053 [2]河北北方学院,河北张家口市075000

出　　处：《中国康复理论与实践》2015年第1期1-4,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：“重大新药创制”科技重大专项(No.2012ZX09102201-016);国家自然科学基金项目(No.81173575;No.81373994);首都医科大学宣武医院院级课题

摘　　要：目的研究莫诺苷对脑缺血再灌注7 d患侧皮层Wnt信号通路相关转录因子神经发生素2(Ngn2)、Pax6、Tbr2表达的影响。方法健康成年雄性Sprague-Dawley大鼠随机分为假手术组,模型组,莫诺苷小、中、大剂量组,每组3只。线栓法制备大鼠大脑中动脉阻塞模型,造模后3 h予莫诺苷30 mg/kg、90 mg/kg、270 mg/kg每天1次灌胃。Western blotting法分析术后7 d皮层Ngn2、Pax6、Tbr2的表达。结果与假手术组相比,模型组皮层Ngn2表达升高(P<0.05);与模型组相比,莫诺苷中、高剂量组Ngn2表达明显升高(P<0.01)。模型组皮层Pax6的表达与假手术组无显著性差异,莫诺苷中、高剂量组Pax6表达升高(P<0.05)。各组间Tbr2的表达无显著性差异。结论莫诺苷能增加脑缺血再灌注后皮层Ngn2、Pax6的表达,提示有促进神经发生的作用。Objective To study the effects of morroniside on the expression of Wnt signaling-related transcription factors neurogenin 2 （Ngn2）, Pax6 and Tbr2 in the ischemic ipsilateral cortex 7 days after cerebral ischemia-reperfusion in rats. Methods 15 male Sprague-Dawley rats were randomly divided into sham group （n=3）, ischemia group （n=3）, and morroniside groups （low, medium and high dosage groups, n=3）. The middle cerebral artery were occluded for 30 min, and re-perfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg 3 hours after operation. The expression of Ngn2, Pax6 and Tbr2 in the ischemic ipsilateral cortex were detected with Western blotting analysis 7 days after operation. Results The expression of Ngn2 increased in the ischemia group compared with the sham group （P〈0.05）, and it further increased the morroniside groups of medium and high dosage compared with the ischemia group （P〈0.01）. There was no significant difference between the ischemia group and sham group in the expression of Pax6, while it increased the morroniside groups of medium and high dosage compared with the ischemia group （P〈0.01）. There was no significant difference among all the groups in the expression of Tbr2. Conclusion Morroniside could increase the expression of Ngn2 and Pax6 in the ischemic ipsilateral cortex 7 days after ischemia-reperfusion in rats, suggesting promoting the neurogenesis after ischemia.

关 键 词：莫诺苷 脑缺血再灌注 转录因子 WNT信号通路 神经发生素2 PAX6 Tbr2 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]