莫诺苷对脑缺血再灌注大鼠皮层梗死周边区基质金属蛋白酶表达的影响  

Effects of Morroniside on Expression of Matrix Metalloproteinases in Peri-infarct Cortex after Cerebral Ischemia-reperfusion in Rats

作　　者：侯虹丽[1] 孙芳玲[2] 艾厚喜[2] 张丽[2] 王文[2] 

机构地区：[1]河北北方学院,河北张家口市075000 [2]首都医科大学宣武医院,北京市100053

出　　处：《中国康复理论与实践》2015年第1期5-8,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金："重大新药创制"科技重大专项(No.2012ZX09102201-016);国家自然科学基金项目(No.81173575;No.81373994);北京市教委科技创新平台项目(No.112219);北京市卫生系统高层次卫生技术人才培养计划项目(No.2011-3-097)

摘　　要：目的研究莫诺苷对大鼠脑缺血再灌注3 d皮层梗死周边区基质金属蛋白酶(MMP)-2、MMP-9表达的影响。方法健康成年雄性Sprague-Dawley大鼠15只随机分为假手术组(n=3),模型组(n=3),莫诺苷小剂量组(n=3)、中剂量组(n=3)及大剂量组(n=3)。采用线栓法制备大鼠大脑中动脉阻塞(MCAO)30 min再灌注模型。术后3 h,莫诺苷各组予莫诺苷30 mg/kg、90 mg/kg、270mg/kg每天1次灌胃。术后3 d,免疫组化染色观察脑缺血皮层梗死周边区MMP-2、MMP-9表达。结果与假手术组比较,模型组皮层梗死周边区MMP-2、MMP-9表达明显著升高(P<0.01)。与模型组比较,莫诺苷各组MMP-2、MMP-9表达明显降低(P<0.01)。结论莫诺苷能降低大鼠脑缺血再灌注3 d皮层梗死周边区MMP-2、MMP-9的表达,从而保护血脑屏障功能。Objective To study the effects of morroniside on the expression of matrix metalloproteinase （MMP）-2 and MMP-9 in the peri-infarct cortex 3 days after cerebral ischemia-reperfusion. Methods 15 male Sprague-Dawley rats were randomly divided into sham group （n=3）, ischemia group （n=3）, and morroniside groups （low, medium and high dosage groups, n=3）. The middle cerebral artery were oc-cluded for 30 min, and re-perfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg 3 hours after operation. The expression of MMP-2 and MMP-9 in peri-infarct cortex were detected with immunohistochemistry staining 3 days after operation. Results The expression of MMP-2 and MMP-9 increased in the ischemia group compared with the sham group （P〈0.01）, and it decreased in all the morroniside groups compared with the ischemia group （P〈0.01）. Conclusion Morroniside could decrease the expression of MMP-2 and MMP-9 in the peri-infarct cortex 3 days after ischemia, suggesting protecting the function of blood-brain barri-er from ischemia.

关 键 词：脑缺血再灌注 莫诺苷 基质金属蛋白酶-2 基质金属蛋白酶-9 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]