谷红注射液对脑缺血再灌注损伤大鼠运动功能障碍的影响  

Effects of Guhong Injection on Motor Dysfunction in Cerebral Ischemia-reperfusion Rats

作　　者：张蕊[1] 范思远 梁珍[1] 纪超[1] 杨楠[1] 刘雁勇[1] 崔丽英 左萍萍[1] 

机构地区：[1]中国医学科学院基础医学研究所药理室,北京市100005 [2]中国医学科学院协和医院,北京市100005

出　　处：《中国康复理论与实践》2015年第1期12-16,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.81100801);菏泽步长制药有限公司项目

摘　　要：目的观察谷红注射液对脑缺血再灌注损伤模型大鼠运动功能障碍的影响。方法 Sprague-Dawley大鼠90只分为假手术组、模型组、乙酰谷酰胺组、红花注射液组和谷红注射液组,采用大脑中动脉闭塞(MCAO)建立脑缺血2 h再灌注损伤模型。术后24 h给药治疗,连续给药14 d。采用Bederson神经缺损症状评分平衡杆测试观察各组运动功能。酪氨酸羟化酶(TH)免疫组化染色观察各组大鼠黑质神经元细胞存活率。结果模型组大鼠的过杆总时间比假手术组明显增加(P<0.01),红花注射液与谷红注射液组过杆总时间较模型组降低(P<0.05)。模型组大鼠缺血侧黑质TH神经元细胞存活率较健侧显著减少(P<0.001),红花注射液和谷红注射液组TH神经元的细胞存活率较模型组明显升高(P<0.01)。结论谷红注射液能显著改善脑缺血再灌注大鼠的运动功能障碍,其保护作用可能与抑制黑质神经元的继发性损伤有关。Objective To evaluate the effects of Guhong Injection on motor dysfunction in rats after cerebral ischemia-reperfusion. Methods The middle cerebral arteries were occluded for 2 hours and re-perfused in Sprague-Dawley rats. They were divided in sham group, model group, Aceglutamide group, Safflowere group and Guhong group, which were intravenously administrated with normal saline, Ace-glutamide, Safflower or Guhong 24 hours after operation, and continued for 14 days. They were tested with the beam-walking test after treat-ment. Tyrosine hydroxylase （TH） immunohistochemical staining was used to investigate the viability of neurons in the substantia nigra. Re-sults The model group spent more time in the beam-walking test than that in the sham group （P〈0.01）, and it decreased in the Safflower group and Guhong group compared with that in the model group （P〈0.05）. The TH-positive neurons decreased in the model rat compared with that in the sham group （P〈0.001）, and increased in both Safflower and Guhong groups compared with that in the model group （P〈0.01）. Conclusion Guhong administration could significantly improve the motor dysfunction in rats after cerebral ischemia-reperfusion, which might be related to provent the neurons from injury in the substantia nigra.

关 键 词：脑缺血再灌注 谷红注射液 运动功能 黑质 酪氨酸羟化酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]