圣女果果皮色素的提取及抗氧化活性研究  

Extraction optimization and antioxidant activity of pigment from cherry tomato peel

作　　者：刘锴栋 袁长春 黎海利 王雁 周鲜娇 赖晓婷 

机构地区：[1]岭南师范学院生命科学与技术学院,广东湛江524048

出　　处：《广东农业科学》2015年第1期83-86,共4页Guangdong Agricultural Sciences

基　　金：国家星火计划项目(2012GA780023;2013GA780081;2013GA780093;2013GA780080);广东省科技计划项目(2013B020304008);广东省科技创新项目(2013KJCX0124);湛江科技攻关计划项目(2012C0203;2013A30210;2011C3106019;2012C3102019);湛江师范学院科研创新团队项目(2013CXTD05);湛江师范学院博士启动项目(ZL09011)

摘　　要：采用乙醇提取法对圣女果果皮色素进行浸提,通过正交设计优化其提取效果,并采用体外模型判断方法,通过分析果皮色素,对超氧阴离子自由基、羟基自由基、DPPH的清除能力研究圣女果果皮色素的抗氧化能力。结果显示,采用90%乙醇+0.5%柠檬酸(5∶1,V/V)的混合液作为浸提溶剂,提取时间为70 min,料液比为1∶14(g/m L),p H值为4.0时,圣女果果皮色素的提取可达到最佳效果。优化后的工艺成本低、操作简单、安全性较高,在工业中有很好的发展前景。圣女果皮色素提取液的浓度与其对超氧阴离子自由基、羟基自由基和DPPH的清除作用呈现一定的正相关关系。The extraction techniques of pigment from cherry tomato peel by solvent method were studied on the basis of single factor test and orthogonal test. The results suggested that the optimal technical condition was as follows： the best extraction technology was extracted solution of 90% ethanol ＋ 0.5% citric acid（5∶1, V/V） in the mixture, the ratio of cherry tomato peel and extraction being 1 ∶14（g/m L）, the p H was 4.0 and the extraction time was 70 min. This extraction technology, taking the ethanol as the extraction solvent, had features of low cost, easy operation and high safety, and had good prospects for development in the industry. The virgin skin pigment extract on the superoxide free radical scavenging effect and hydroxyl free radical scavenging effect and DPPH radical was positively correlated with concentration.

关 键 词：圣女果果皮 正交试验 色素 抗氧化活性 

分 类 号：S667[农业科学—果树学]