核盘菌基因组微卫星序列分析  

SSRs analysis of genome in Sclerotinia sclerotiorum

作　　者：张悦[1] 李丹 陈子牛[1] 王丽燕[1] 

机构地区：[1]昆明学院生命科学与技术系,云南昆明650214 [2]云南交通技师学院,云南安宁650300

出　　处：《广东农业科学》2015年第1期128-132,共5页Guangdong Agricultural Sciences

基　　金：云南省科技厅应用基础研究计划青年项目(2012FD046)

摘　　要：利用已经公布的核盘菌基因组测序结果,对已有的核盘菌基因组中SSR的类型、大小及分布等数据进行统计分析。在核盘菌基因组内发现1 693个SSR序列,其中出现频率最高的为6碱基和4碱基的SSR序列,分别达到577次和449次,1碱基、3碱基和5碱基的SSR则分别出现97、180、57次。其中有340个SSR序列出现在308个基因内。大多数基因(284个基因)都只含有1个SSR,占含SSR基因总数的92.21%;17个基因中含有2个SSR,6个基因中含有3个SSR,含4个SSR的基因只有1个。在基因序列内,出现最多的是3碱基与6碱基的SSR,这表明较之基因间区,在选择压力下基因内SSR多趋向于密码子的整数倍,这与其他物种的分析结果一致。为了解含有较多SSR序列与核盘菌基因功能的关系,将含2个以上SSR的基因预测蛋白序列根据NCBI网站CDD软件进行比对发现,24个基因只有6个具有保守的蛋白结构域,且这些结构域多与转录、DNA修复有关。This study analyzed the SSRs in the genome of Sclerotinia sclerotiorum according to the genome sequence data from public database. Totally we found 1 693 SSRs, most of them were 6 and 4 bases SSRs which appeared 577 and449 times, respectively. 1 base, 3 bases and 5 bases SSRs appeared 97, 180 and 57 times, respectively. There were 340 SSRs from 308 putative gene sequences, 284 of them contained only 1 SSR, accounting for 92.21% of the total; 17 of genes contained 2 SSRs, 6 genes contained 3 SSRs and only 1 gene contained 4 SSRs. Compared with SSRs from whole genome,the SSRs with most frequency were 3 and 6 bases SSRs, which meant that under the selection pressure, the SSRs in gene sequences were tend to be integer multiples of gene code number 3, this was consistent with the research on other species.To analyze the functions of the genes contained more than 2 SSRs of S. sclerotiorum, we blast all these 24 genes by CCD software from NCBI. Among them, only 6 genes contained conserved proteins domains and most of these domains were related to transcription and DNA reparation.

关 键 词：微卫星序列 核盘菌 基因组 分子标记 

分 类 号：S432.1[农业科学—植物病理学] Q933[农业科学—农业昆虫与害虫防治]