新型肿瘤靶向药物TMTP1-DKK应用于宫颈癌治疗的初步研究  

Anti-tumor effects of the peptide TMTP1-DKK on different cervical cancers

作　　者：魏睿[1] 李飞[1] 张振中[1] 程腾[1] 奚玲[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉430030

出　　处：《实用药物与临床》2015年第1期1-5,共5页Practical Pharmacy and Clinical Remedies

基　　金：国家自然科学基金面上项目(81172468);华中科技大学研究生创新创业基金项目(01-09-070115)

摘　　要：目的探讨TMTP1-DKK在治疗高转移宫颈癌的潜在应用价值。方法选择高转移和低转移配对宫颈癌细胞系SiHa和C-33A,Transwell试验分别确证其转移能力;Rhodamine标记TMTP1,免疫荧光检测Rhodamine-TMTP1对SiHa、C-33A的亲和能力;CCK8检测TM TP1-DKK和TM TP1-VK处理后对Si Ha和C-33A细胞的杀伤率,Transewell检测TMTP1-DKK和TMTP1-VK处理后对SiHa细胞侵袭能力的影响。结果 SiHa细胞侵袭能力强于C-33A,TMTP1亲和实验显示TMTP1对高转移细胞系Si Ha亲和力明显高于C-33A,TMTP1-DKK对Si Ha生存率影响明显强于C-33A,差异有统计学意义(P<0.05)。结论 TM TP1-DKK能特异性地杀伤高侵袭能力的宫颈癌细胞Si Ha,但对低转移能力细胞C-33A杀伤效应不明显。提示TMTP1-DKK可以作为高转移宫颈癌靶向化疗药物的可能性。Objective To investigate the remarkable specificity and anti-tumor ability of peptide TMTP1-DKK on highly metastatic cervical tumors in vitro. Methods Cervical cancer cells SiHa and C-33 A that had different metastatic capacities were used. The cell invasion was investigated using the Transwell system. Rhodamine was coupled to the peptides TMTP1. Immunofluorescence assay was carried out to determine the specific binding capacities of the TMTP1,SiHa and C-33 A. The inhibition rates of cells growth was measured by CKK-8 assay. The SiHa and C-33 A cells were treated with different concentrations of TMTP1-DKK and TMTP1-VK in vitro,and the biological behavior changes of cells were assessed by migration and invasion through Transwell assay. Results Cervical cancer cell SiHa had higher metastatic ability than C-33 A. Rhodamine-TMTP1 bound specifically to highly metastatic tumor cell line,SiHa,however,Rhodamine-TMTP1 did not bind to thenometastatic cervical cancer cell C-33 A. TMTP1-DKK showed higher anti-tumor effects on highly metastatic SiHa compared to C33 A,and the difference was significant（ P 0. 05）.Conclusion TMTP1-DKK can inhibit the proliferation of highly metastatic cervical cancer cell SiHa,but it has little effect onnometastatic cervical cancer cell C-33 A. These results suggest TMTP1-DKK may be a powerful candidate therapeutic agent for metastatic tumors.

关 键 词：TMTP1 宫颈癌 肿瘤转移 靶向治疗 

分 类 号：R737.33[医药卫生—肿瘤]