不同时期成釉细胞氟损伤程度差异的动物实验研究  

The injury diversity of fluoride to ameloblasts at different times in rats

作　　者：张凯强[1,2] 张颖[1,2] 顾何锋[1,2] 马林[1,2] 程睿波[1,2] 刘璐[1,2] 张思宇[1,2] 

机构地区：[1]中国医科大学口腔医学院口腔预防科 [2]辽宁省口腔医学研究所口腔预防教研室,辽宁沈阳110002

出　　处：《口腔医学》2014年第12期881-884,共4页Stomatology

基　　金：国家自然科学基金资助项目(81072245);辽宁省自然科学基金资助项目(20102278)

摘　　要：目的检测GRP78和BCL-2在氟中毒大鼠成釉细胞中的表达和分布,探索不同时期成釉细胞氟损伤程度差异。方法选择20只Wistar大鼠,饲喂氟浓度为75 mg/L的自来水,8周后处死,通过HE染色和免疫组化技术观察大鼠成釉细胞形态,以及GRP78和BCL-2在氟中毒大鼠不同时期成釉细胞中的表达。结果 GRP78在成釉细胞分泌前期和分泌期表达高于转换期和成熟期,BCL-2在分泌期和转换期表达最高,在分泌前期和成熟期表达下降,各组间具有统计学差异(P<0.05)。结论处于不同时期的成釉细胞对氟损伤程度存在差异。Objective To study the expression of GRP78 and BCL-2 in ameloblasts of fluorosis rats and to explore the injury diversi- ty of fluoride to ameloblasts at different stages. Methods 20 Wistar rats were selected and fed with fluoride water. 8 weeks later,they were sacrificed. Immunobistochemistry and HE staining were used to detect the changes of amelnblast and the expression of GRP78, BCL-2 in rat incisors. Metamorphic microscope images analysis system and SPSS13.0 software were used to analyze the data. Results The expression of GRP78 in ameloblast pre-sccretory phase and secretory phase was higher than that in transformation period and ma- ture period. BCL-2 had the highest expression in secretory phase and transition period, however,it declined in pre-secretory stage and mature stage. The differences between groups were statistically significant （ P 〈 0.05 ）. Conclusions The injury of fluoride to amelo- blasts at different times is different.

关 键 词：氟 成釉细胞 损伤差异 

分 类 号：R361.3[医药卫生—病理学]