老龄鼠实验性牙周炎的免疫特点  

Characteristics of the immune status in aged mice with experimental periodontitis

作　　者：靳赢[1] 王琳源[1] 刘迪昕[1] 林晓萍[1] 

机构地区：[1]中国医科大学附属盛京医院,口腔科,辽宁沈阳110004

出　　处：《上海口腔医学》2014年第6期669-674,共6页Shanghai Journal of Stomatology

基　　金：高等学校博士学科点专项科研基金(20112104110013);辽宁省科学技术计划项目(2012225015);沈阳市科技计划项目(F13-220-9-28)~~

摘　　要：目的 :通过口腔涂抹牙周致病菌的方法建立小鼠牙周炎模型,比较低龄小鼠与老龄小鼠牙周炎发病免疫状态差异,分析Th17/Treg细胞相关因子在不同年龄牙周炎小鼠中的表达情况。方法:采用实体显微镜评价牙周骨吸收的程度,H-E染色法观察牙周组织病变的炎细胞浸润情况,TRAP染色法观察牙周骨组织表面破骨细胞浸润情况,实时定量PCR检测牙龈组织中Th17/Treg细胞相关因子TGF-β1、IL-10、IL-17A、RANKL m RNA的表达水平。采用SPSS17.0软件包对数据进行统计学分析。结果:实验4周后,牙周炎组与正常对照组相比,釉-牙骨质界至牙槽嵴顶的距离显著增加(P<0.01),老龄组小鼠釉-牙骨质界至牙槽嵴顶的距离比低龄组小鼠显著增加(P<0.01);牙周组织中大量炎细胞浸润,牙周袋加深;牙周组织中TGF-β1、IL-10 m RNA的表达水平显著降低(P<0.01),IL-17A、RANKL m RNA表达水平显著增高(P<0.01)。结论:老龄小鼠牙周炎中炎症介质的表达存在异常状态,比低龄小鼠牙周组织病损状态加剧,提示牙周炎的炎症状态可能不仅与其分泌的免疫抑制性细胞因子表达降低有关,更与其增龄性相关。PURPOSE： To establish mice model with periodontitis by oral infection with Porphyromonas gingivalis, simulating human periodontal disease. The immune status of youth and aged mice were compared, and the cytokines expression of regulatory T cells and Thl7 cells in this model were analyzed. METHODS： Entity microscope was used to evaluate the extent of periodontal bone resorption. H-E staining was used to observe infiltration of inflammatory cells in periodontal lesions. TRAP staining was used to observe osteoclastes in alveolar bone. The expression levels of TGF-β1, IL-10, IL-17A and RANKL mRNA in periodontal tissues were detected by real-time quantitative PCR. SPSS 17.0 software package was used for statistical analysis. RESULTS： At 4-week after infection, the distance of cemento-enamel junction to the alveolar crest was significantly increased in the periodontal disease group compared with the normal control group （P〈0.01）; In the aged mice, the cemento-enamel junction to the alveolar crest distance significantly increased compared with youth mice （P〈0.01）. Periodontal tissues had inflammatory cell infiltration, and deep periodontal pockets. Moreover, the expression levels of TGF-131 and IL-10 mRNA were significantly decreased （P〈0.01） and the expression levels of IL-17A, RANKL mRNA significantly increased （P〈0.01）. CONCLUSIONS： The expression of inflammation mediator is abnormal in aged mice with more serious periodontal lesions than youth mice. It is suggested that the inflammatory status of periodontitis not only has a relationship with the decreased expression of inhibitory cytokines, but also relates to aging.Supported by Higher Institute School Special Research Fund for Doctoral Program （20112104110013）, Research Fund of Science and Technology Plan of Liaoning Province （2012225015） and Research Fund of Science and Technology Plan of Shenyang City （F13-220-9-28）.

关 键 词：牙周炎 动物模型 骨吸收 年龄 免疫 

分 类 号：R781.42[医药卫生—口腔医学]