新型牙髓治疗材料iRoot BP Plus对人牙龈成纤维细胞的细胞毒性  

Cytotoxicity of a novel endodontic treatment material i Root BP Plus to human gingival fibroblasts

作　　者：史爽[1] 包志凡[1,2] 陈旭[1,2] 张丹丹[1] 

机构地区：[1]中国医科大学口腔医学院儿童口腔科 [2]辽宁省口腔医学研究所儿童口腔医学研究室,辽宁沈阳110002

出　　处：《上海口腔医学》2014年第6期681-684,共4页Shanghai Journal of Stomatology

基　　金：辽宁省科学技术计划项目(2012225015);沈阳市科学技术计划项目(F12-277-1-65)~~

摘　　要：目的：比较新型牙髓治疗材料i Root BP Plus与矿物三氧化物凝聚体（mineral trioxide aggregate,MTA）对人牙龈成纤维细胞的细胞毒性。方法：采用四甲基偶氮唑盐（methyl-thiazol-tetrazolium,MTT）法测定上述2种材料不同浸提时间（1、3、7 d）的浸提液原液及不同浓度稀（1：2、1：5）释液对体外培养的人牙龈成纤维细胞增殖的影响。细胞增殖率以x±s表示,采用SPSS20.0软件包对数据进行析因设计的方差分析。结果：i Root BP Plus和MTA的浸提液原液及不同浓度稀释液作用后的细胞增殖率界于77.31%~113.82%,细胞毒性分级为0或1级,评价结果为无细胞毒性。不同时间点、不同稀释度下,2种材料对体外培养的人牙龈成纤维细胞增殖率的影响无显著差异（F浓度＊时间＊材料=1.393,P=0.256）。结论 ：i Root BP Plus与MTA均对体外培养的人牙龈成纤维细胞无细胞毒性。PURPOSE： To compare the cytotoxicity of a novel endodontic treatment material iRoot BP Plus and mineral trioxide aggregate （MTA） to human gingival fibroblasts in vitro. METHODS： Cultured human gingival fibroblasts were exposed to multiple concentrations of material elutes （no dilution, 1：2 dilution, and 1：5 dilution）. The test material samples were immersed and incubated in the culture medium for 1, 3 or 7 days at 37℃. The proliferation rate was evaluated using methylthiazol tetrazolium （MTT） assay. Cell relative growth rates were presented as x-＋s. The data was statistically analyzed by factorial design ANOVA using SPSS 20.0 software package. RESULTS： Cell relative growth rates of the eluates of iRoot BP Plus and MTA in different concentrations ranged from 77.31% to 113.82%. The eytotoxicity grade of both materials was 0 or 1 （no cytotoxicity）. There was no significant difference in the relative growth rate in different concentrations of iRoot BP plus and MTA eluates under different elution times （Fconcentration＊tirne＊material=1.393, P=0.256）. CONCLUSIONS： Both iRoot BP Plus and MTA exhibit minimal level of cytotoxicity.

关 键 词：iRoot BP PLUS 矿物三氧化物凝聚体 细胞毒性 人牙龈成纤维细胞 

分 类 号：R783.1[医药卫生—口腔医学]