人工麝香高效液相色谱特征图谱分析  

High Performance Liquid Chromatography Characteristic Spectrum of Artificial Musk

作　　者：张萍[1] 肖宣[2] 张南平[1] 肖新月[1] 章菽 魏锋[1] 于德泉[2] 

机构地区：[1]中国食品药品检定研究院中药民族药检定研究所,北京100050 [2]中国医学科学院北京协和医学院药物研究所天然产物室,北京100050 [3]北京联馨药业有限公司技术部,北京102628

出　　处：《中国医学科学院学报》2014年第6期587-590,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的 采用高效液相色谱法建立人工麝香特征图谱,提高完善人工麝香质量控制方法.方法 采用HiQ Sil C18 （4.6 mm×250 mm）色谱柱;以甲醇-水（90∶10）为流动相,柱温为25℃,检测波长为254 nm.采用国家药典委员会推荐使用的《中药色谱指纹图谱相似度评价系统》对10批人工麝香样品进行相似度分析.结果 10批人工麝香样品相似度为0.999 ～1.000.结论 人工麝香样品批间重现性较好,工艺稳定.建立的人工麝香特征图谱方法简便、准确、可靠,可作为人工麝香的质量控制方法.Objective To determine the high performance liquid chromatography （HPLC） character- istic spectrum of artificial musk. Methods HPLC was performed on a HiQ Sil C18 analytical column （4. 6 mm × 250 mm） with the mobile phase of methanol/water （90：10 v/v） ; the detection wavelength was 254 nm and the column temperature was maintained at 25 ℃. The similarities among 10 batches of artificial musk samples were anlysised in accordance with the System for Evaluating the Similarities among the Chromatographic Fingerprints of Traditional Chinese Drugs, which is recommended by Chinese Pharmacopoeia Commission. Result The similar- ities among these 10 batches of artificial musk ranged between 0. 999 and 1. 000. Conclusion HPLC is a con- venient, accurate and reliable technique for establishing the specific spectum of artificial musk and therefore can be used for the quality control of this product.

关 键 词：人工麝香 特征图谱 质量控制 

分 类 号：R917[医药卫生—药物分析学]