高糖和高脂饮食对小鼠肥胖和内脏脂肪的影响  

Effect of High-fat or High-glucose Diet on Obesity and Visceral Adipose Tissue in Mice

作　　者：唐丽丽[1,2,3] 汤晓涵[1,2,3] 李霞[1,2,3] 俞海波[1,2,3] 谢志国[1,2,3] 刘新元[1,2,3] 周智广[1,2,3] 

机构地区：[1]中南大学湘雅二医院内分泌科 [2]中南大学代谢内分泌研究所 [3]中南大学糖尿病中心糖尿病免疫学教育部重点实验室国家代谢性疾病临床医学研究中心,长沙410011

出　　处：《中国医学科学院学报》2014年第6期614-619,共6页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(81070672)~~

摘　　要：目的探讨高脂和高糖饮食对小鼠肥胖和内脏脂肪的不同影响。方法将4周龄C57BL/6小鼠按随机数字表法分为正常饮食组、高脂饮食组和高糖饮食组,喂养至20周龄,比较各组体重、附睾旁脂肪重量、瘦素、脂肪肝程度、附睾旁脂肪组织巨噬细胞及其亚型的浸润和单核细胞趋化蛋白-1 mRNA的表达。结果 20周时高脂饮食组小鼠体重、附睾旁脂肪重量、瘦素水平较正常饮食组均显著升高(P均<0.05),油红O染色显示高脂饮食组肝脏脂肪浸润明显。20周时高糖饮食组在体重、附睾旁脂肪重量、瘦素水平与对照组相比差异均无统计学意义(P均>0.05)。高脂饮食组小鼠脂肪内巨噬细胞的浸润增加,且M2的比例较高糖饮食组显著降低(P<0.05)。高脂饮食组小鼠附睾旁脂肪中单核细胞趋化蛋白-1 mRNA的表达较正常饮食组显著上调(P<0.05),而高糖饮食组与正常饮食组相比差异无统计学意义(P>0.05)。结论高脂饮食,而不是高糖饮食,可导致肥胖和内脏脂肪组织内巨噬细胞的炎症浸润。Objective To investigate the effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in C57BL/6 mice.Methods Four-week-old C57BL/6 mice were allocated into normal diet group,high-fat diet group,and high-glucose diet group according to the random number table until 20 weeks old.Body weight,epididymal adipose tissue weight,blood leptin,fat infiltration in liver,M1/M2 macrophage subtypes,and monocyte chemoattractant protein-1 mRNA in epididymal adipose tissues were measured.Results Compared with normal diet group,body weight,epididymal adipose tissue weight,and leptin concentration in high fat diet group at 20 weeks were significantly increased （P 〈 0.05）,and oil red O staining showed more prominent adipocyte infiltration in liver in high-fat diet group than those in normal diet and high-glucose diet group.However,no apparent differences were seen in high-glucose diet group at 20 weeks in terms of body weight,epididymal adipose tissue weight and leptin concentration.In high-fat diet group,the macrophages infiltration in epididymal adipose tissue increased with time and the percentage of M2 macrophage decreased in highfat diet group than that in high-glucose diet group （P 〈 0.05）.Compared with normal diet group,monocyte chemoattractant protein-1 mRNA expression increased significantly in high-fat diet group （P 〈 0.05）.In highglucose group,however,no significant differences were discerned （P 〉 0.05）.Conclusion High-fat diet,rather than 60% high glucose diet,will lead to obesity and macrophage infiltration in adipose tissues.

关 键 词：脂肪组织 高脂饮食 高糖饮食 巨噬细胞 肥胖 

分 类 号：R589.2[医药卫生—内分泌]