白灵菇菌种退化的快速鉴别方法  

Methods for rapid differentiation of degenerate strains for cultivated Pleurotus nebrodensis

作　　者：李莎[1,2] 刘宇[2] 马康[2] 马元伟[2] 李明[1] 许峰[2] 

机构地区：[1]河北农业大学园艺学院,河北保定071000 [2]北京市农林科学院植物保护环境保护研究所,北京市食用菌工程技术研究中心,北京100097

出　　处：《生物技术》2014年第6期66-68,共3页Biotechnology

基　　金：现代农业产业技术体系北京市食用菌创新团队(编号:PXM2013-036204-00069)资助

摘　　要：[目的]建立白灵菇退化菌种的检测方法。[方法]退化的白灵菇白10菌株和提纯复壮的自交45×49菌株为试验材料,分别采用YBLB试剂、LBL液体摇瓶培养和悬浮液脱色培养基以提纯复壮的菌株为对照,对退化的菌株进行鉴别。[结果]YBLB、LBL两种方法中正常菌株均能使试剂或培养基脱色,退化菌株则不能,从而可定性区分出正常菌株与退化菌株,悬浮液脱色培养基方法定量测定了脱色培养基中的脱色率,退化菌株白10的脱色率仅为54.7%,而正常菌株脱色率高达95.44%。[结论]成功地建立了白灵菇退化菌种定性及定量的快速鉴别方法,退化菌株的脱色率D<60。[ Objective] The aim of this study was to establish the methods for differentiation of degenerate strains for cultivated Pleurotus nebrodensis. [ Methods ] Degenerate strain and normal strain of P. nebrodensis were inoculated in YBLB reagent or LBL liquid media and resuspension media containing bromothymol blue, then was cultured till decolorizing of the normal strains. [ Results ] The former two： methods tested can differential the degernerated strains from the normal ones efficiently. Decolorizing ratio in the resuspension media was caculated, as 54. 7 % for the degernerated strains and 95.44% for the normal ones, respectively. [ Conclusion] Methods for rapid differentiation of degenerate strains for cultivated P. nebrodensis was established and the decolorizing ratio reached D 〈 60.

关 键 词：BTB 脱色反应 菌种退化 鉴别 白灵菇 

分 类 号：S939.5[农业科学—渔业资源]