淡紫拟青霉固态发酵工艺优化  

Optimization of the solid state fermentation process of Paecilomyces lilacinus

作　　者：谷军[1] 张晓彦[1] 沙长青[1,2] 

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院,黑龙江哈尔滨150001

出　　处：《生物技术》2014年第6期94-98,共5页Biotechnology

基　　金：黑龙江省财政基本科研业务费专项("固体发酵生产系统研制及在淡紫拟青霉生产上的应用";编号:CZ14BGJV06)资助

摘　　要：[目的]在淡紫拟青霉固体发酵生产过程中,提高淡紫拟青霉分生孢子产量。[方法]通过研究筛选发酵原料和优化固体发酵培养基,控制发酵工艺条件等方法,提高了固体发酵淡紫拟青霉的分生孢子产量。[结果]通过研究固体发酵过程,得出玉米粉作用显著,能显著提高固体发酵淡紫拟青霉的分生孢子数,可达到1.31×1010CFU/g。[结论]该淡紫拟青霉的最优培养基为:麸皮72.5%、玉米粉15%、豆饼粉10%、稻壳2%、硫酸铵0.5%。生产工艺为原料与水的比例为1:0.6,发酵温度为26℃,发酵培养时间为8d。[ Objective ] Improve production of Paecilomyces lilacinus spores number during P. lilacinus solid state fermentation production process. [ Methods] Fermentation feedstock solid fermentation medium, fermentation conditions control and other improve solid fermentation P. lilacinus spore number methods were studied. [ Results ] During the fermentation process, corn flour can significant increase the number of P. lilacinus spores number, it can be reached to 1.31 ×10^10 CFU/g. [ Conclusion]The best medium optimization for P. lilacinus were 72.5% bran, 15% corn flour, 10% soybean meal, 2% rice husk and 0.5% ammonium sulfate. The proportion of production raw materials and water were 1 ： 0.6, fermentation temperature were controlled at 26 ℃, fermentation period was 8 days.

关 键 词：淡紫拟青霉 固态发酵 正交设计 分生孢子数 

分 类 号：Q815[生物学—生物工程]