多孔性碳酸化羟基磷灰石支架材料体外可吸收性测定  

Bioresorption of Porous Carbonated Hydroxyapatite In Vitro

作　　者：周琳怡 乔亚南[1] 刘楠[1] 曹寅[2] 张磊[1] 

机构地区：[1]同济大学口腔医学院修复科,口腔生物医学及转化医学实验室,上海200072 [2]常州市口腔医院,江苏常州213000

出　　处：《口腔颌面外科杂志》2014年第6期425-428,共4页Journal of Oral and Maxillofacial Surgery

基　　金：国家大学生创新性实验计划项目(1504107007)

摘　　要：目的:通过体外实验对不同组分多孔碳酸化羟基磷灰石(PCHA)支架材料的可吸收性进行评价。方法 :将制得的不同孔隙率、不同碳酸根含量的15种PCHA样本(n=3),置于100 m L、p H=4.0的醋酸钠缓冲液(模拟破骨细胞造成的酸性环境)中;对照组为羟基磷灰石(HAP)及脱脂处理过的牛松质骨。每组材料均分别在浸泡1、4、8 h或1、2、3、4、5、7 d后,取0.1 m L浸提缓冲液进行Ca2+浓度测定,以评价材料的可吸收性。结果 :各PCHA实验组和牛松质骨对照组的Ca2+浓度,均随浸泡时间增加而增高,HAP对照组无明显变化。随着碳酸化程度和孔隙率的增加,PCHA的溶解速率也随之增加。其中7-40组、7-60组、8-40组、8-60组、8-70组、9-70组在各时间点测得的PCHA溶出钙离子浓度,与对照牛松质骨对照组差异均无统计学意义(P>0.05),以上几组PCHA与牛松质骨具有最相似的可吸收性。结论:PCHA材料表现出良好的可吸收性,碳酸化的程度和孔隙率的大小是影响其可吸收性的重要因素。Objective： To evaluate the bioabsorbability of a novel porous carbonated hydroxyapatite （PCHA） in vitro. Methods： 15 types of PCHA samples （n=3）, with different carbonate contents and porosities, were immersed at 100 mL, pH 4 acetic acid buffer solution for extraction. Hydroxyapatite （HAP） and degreased cattle bone blocks were used as control. Then 0.1 mL extracted solution was taken out at 1 h, 4 h, 8 h, 1 d, 2 d, 3 d, 4 d, 5 d and 7 d respectively for calcium analysis . Concentration curves were plotted to evaluate the absorbability of the samples. Results： Calcium concentrations in PCHA samples and cattle bone blocks all increased over time, while no obvious variations showed in HAP group. PCHA samples with higher carbonate contents and porosities showed faster calcium ion release. Calcium concentration curves in groups of 7-40, 7-60, 8-40, 8-60, 8-70, 9-70 showed better consistency with those of cattle bone blocks, indicated they had similar bioabsorbability. Conclusion： PCHA has good bioabsorbability in vitro, and the absorbability can be affected by degrees of carbonation and porosity.

关 键 词：碳酸化羟基磷灰石 多孔性 可吸收性 组织工程 

分 类 号：R783.1[医药卫生—口腔医学]