外周血游离DNA中Septin9基因甲基化在结直肠癌筛查中的意义  

Significance of Septin9 gene methylation detection of plasma circulation DNA in colorectal cancer screening

作　　者：康倩[1] 金鹏[1] 杨浪[1] 王昕[1] 安贺娟[1] 刘丽丽[1] 李娜[1] 盛剑秋[1] 

机构地区：[1]北京军区总医院消化内科,100700

出　　处：《中华医学杂志》2014年第48期3839-3841,共3页National Medical Journal of China

基　　金：军队保健专项科研课题（12BJZ04）

摘　　要：目的 探讨血浆游离DNA中Septin9基因甲基化状态在中国人群结直肠癌筛查中的作用.方法 北京军区总医院消化内科内镜中心自2013年9月至2014年2月接受结肠镜检查的患者,按照肠镜检查与病理诊断结果分为结直肠癌组（80例）与未见异常的对照组（52例）,采用单盲法应用荧光定量PCR方法进行外周血游离DNA中Septin9基因甲基化状态检测,并通过胶体金免疫化学法进行粪隐血检测,对比Septin9甲基化与粪隐血检测在筛查结直肠癌中的优越性.结果 Septin9基因甲基化筛查结直肠癌的敏感度为75.0％ （95％ CI：64.7％ ～ 83.6％）,特异度为98.1％（95％ CI：90.9％ ～99.9％）;优于粪隐血检测筛查结直肠癌（敏感度：79.5％比53.8％,P＜0.05）.结论 检测血浆中Septin9基因甲基化状态可作为一种新的无创性早期筛查结直肠癌的方法.Objective To explore the role of detecting the methylation status of gene Septin9 （SEPT9） in plasma for colorectal cancer screening in Chinese population.Methods Patients were collected from Beijing Military General Hospital since September 2013 to February 2014.The performance of SEPT9 assay was validated in a single-blind study of 80 cases with colonoscopy and pathologically verified colorectal cancer and 52 normal controls.The detection of Septin9 gene methylation in peripheral blood was performed by fluorescence quantitative polymerase chain reaction （PCR）.And immunoassay fecal occult blood test was conducted to compare the superiority of methylated Septin9 for screening colorectal cancer.Results The Septin9 assay successfully identified 75.0％ （95％ CI：64.7％-83.6％） of cancers at a specificity of 98.1％ （95％ CI：90.9％-99.9％）.And it was superior to fecal occult blood screening for colorectal cancer （sensitivity 79.5％ vs 53.8％,P ＜ 0.05）.Conclusion Determination of SEPT9 methylation status is an innovative non-invasive plasma screening test for colorectal cancer.

关 键 词：结直肠肿瘤 DNA甲基化 基因 Septin9 

分 类 号：R735.3[医药卫生—肿瘤]