PURα通过上皮间叶转化促进食管癌细胞KYSE 510的侵袭和转移  

Purine-rich element binding protein alpha promotes invasion and migration of esophageal squamous cell carcinoma KYSE 510 cells by inducing epithelialmesenchymal transition

作　　者：郭志敏[1] 李薇[1] 赵晓航[1] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤研究所分子肿瘤学国家重点实验室,北京市100021

出　　处：《世界华人消化杂志》2014年第36期5579-5586,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81372591;国家高技术发展计划"863"基金资助项目;No.2012AA020206;国家重点基础研究发展计划"973"基金资助项目;No.2011CB910703~~

摘　　要：目的:富含嘌呤元件结合蛋白α(purine-rich element binding protein alpha,PURα)是调控细胞周期和恶性转化的关键因子.本研究旨在探讨在食管鳞状细胞癌中转录因子PURα蛋白的多种功能及其相关机制.方法:构建过表达PURα蛋白的食管癌细胞KYSE510-PURα和p CMV6空载体转染的对照食管癌细胞KYSE 510-p CMV6.用蛋白免疫印迹和细胞免疫荧光分析检测过表达PURα蛋白的食管癌细胞和对照食管癌细胞中上皮间叶转变相关蛋白的表达差异,如E-钙黏蛋白和波形蛋白等;采用细胞划痕实验和Transwell小室检测食管癌细胞的侵袭转移能力.结果:成功构建过表达PURα的食管癌细胞.蛋白免疫印迹和免疫荧光分析表明,与对照细胞相比,过表达PURα的食管癌细胞中E-钙黏蛋白的表达减少,N-钙黏蛋白、波形蛋白和Snail蛋白表达升高.引人注目的是,过表达PURα的食管癌细胞的划痕愈合能力增强,侵袭性显著增加.与此同时,细胞形态发生了纤维样改变,呈现上皮间叶转化表型.结论:PURα能通过调控食管鳞癌细胞上皮间叶转化而促进肿瘤细胞的侵袭转移能力.AIM： To investigate the role of purine-rich element binding protein alpha （PURα） in the invasion and migration of esophageal squamous cell carcinoma （ESCC） KYSE 510 cells and the underlying mechanisms. METHODS： An ESCC cell line overexpressing PURα （KYSE 510-PURα） was established, and the expression levels of epithelial-mesenchymal transition associated proteins were determined by comparing with control cells transfected with an empty vector of pCMV6 （KYSE 510-pCMV6）. The expression of E-cadherin and vimentin was analyzed by Western blot and immunofluorescent staining. The capabilities of invasion and migration of cancer cells were assessed via transwell and wound healing assays. RESULTS： PURα was overexpressed in KYSE 510 cells transfected with the pCMV6- PURα vector. The expression of E-cadherin was reduced, and that of Vimentin, N-cadherin and Snail was increased in KYSE 510-PURα cells. Most strikingly, the cell morphology was changed as fibroblasts and the abilities of migration and invasion were altered. CONCLUSION： Our data suggest that regulation of PURα expression in ESCC cells may induce esophageal epithelial-mesenchymal transition.

关 键 词：富含嘌呤元件结合蛋白α 食管鳞状细胞癌 上皮间叶转化 

分 类 号：R735.1[医药卫生—肿瘤]