SA-3大孔树脂纯化甜茶素的研究  

Studies on Purification of Rubusoside in Sweet Tea by SA-3 Macroporous Resins

作　　者：吴婕[1] 鲍晨阳 杜莹[2] 廖莉玲[2] 

机构地区：[1]贵州师范学院化学与生命科学学院,贵州贵阳550018 [2]贵州师范大学化学与材料科学学院,贵州贵阳550001

出　　处：《食品研究与开发》2014年第23期36-39,共4页Food Research and Development

基　　金：贵州省科学技术厅中药现代化攻关项目(黔科合ZY字【2012】3012号);贵阳市科技局现代药业计划项目(筑科合同[2012204]号);贵州师范学院2013年度校级科研一般项目(甜茶叶中甜茶苷的提取)

摘　　要：通过比较9种大孔树脂对甜茶素的吸附和解吸效果,筛选出适合甜茶素分离纯化的树脂,并对其纯化工艺条件进行了探讨。结果表明,SA-3型大孔吸附树脂最适合甜茶素纯化。最佳吸附条件为:上柱液甜茶素浓度控制在1.00 mg/m L^3.00 mg/m L,上样流速3 BV/h,上样液体积控制在17 BV以内;最佳洗脱条件为:70%乙醇3 BV/h洗脱,洗脱体积为13 BV。经SA-3型大孔吸附树脂纯化纯度甜茶素可达到45%左右。The SA-3 was optimized from 9 types of macroporous resins by comparing their static adsorption and desorption performances with rubusoside in Sweet tea. The purifying conditions of rubusoside from Sweet tea by SA-3 macroporous resins was investigated. The results showed that SA-3 macroporous resins was the best one. The optimal adsorption process conditions were as follows：concentration of sample 1.00 mg/mL-3.00 mg/mL;3 BV/h adsorption velocity;17times the volume of resin as sample volume;the best desorption conditions：70%ethanol and 13 times the volume of resin as eluent. The content of rubusoside purified by SA-3 macroporous resin was up to 45%.

关 键 词：大孔树脂 SA-3 甜茶素 

分 类 号：TS202.3[轻工技术与工程—食品科学]