菊苣干预高尿酸血症鹌鹑尿酸及相关代谢酶活性研究  

Effect of Chicory on Uric Acid and Uricopoiesis Metabolic Enzymes Activities of Hyperuricemia Quail

作　　者：黄胜男[1] 林志健[1] 张冰[1] 赓迪[1] 牛红娟[1] 朱春胜[1] 王雪洁[1] 孙博喻[1] 

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《中药新药与临床药理》2015年第1期9-13,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：教育部高等学校博士学科点专项科研基金(20130013120001;20120013130002);北京中医药大学科研创新团队(2011-CXTD-014);北京中医药大学自主课题(2013-QNJSZX008)

摘　　要：目的探讨菊苣对高尿酸血症(HUA)鹌鹑尿酸及相关代谢酶活性的影响,阐释菊苣防治HUA的可能机制。方法将60只鹌鹑,按体质量随机分为5组,即正常组,模型组,苯溴马隆组(20 mg·kg-1·d-1),菊苣高、低剂量组(10,5 g·kg-1·d-1),每组12只。除正常组喂饲鹌鹑普通饲料外,其余各组均给予高嘌呤饲料(普通饲料拌入酵母干粉15 g·kg-1·d-1)复制HUA模型。以菊苣治疗28 d,动态观察鹌鹑尿酸(uric acid,UA)水平及UA生成代谢酶活性的变化。结果给药7,14,21,28 d,菊苣高、低剂量组可显著降低鹌鹑UA水平(P<0.05);不同程度地抑制UA生成代谢酶5'-核苷酸酶(5'-NT)、腺苷脱氨酶(ADA)、嘌呤核苷磷酸化酶(PNP)、鸟嘌呤脱氨酶(GD)、黄嘌呤氧化酶(XO)的活性(P<0.05,P<0.01)。结论菊苣可有效降低鹌鹑HUA模型UA水平,可能与其降低UA代谢酶5'-NT、ADA、PNP、GD、XO的活性有关。Objective To investigate the effect of chicory on uric acid and uricopoiesis metabolic enzymes activities in hyperuricemia quail and to explore the possible mechanism of chicory in preventing and treating hyperuricemia. Methods Sixty quails were evenly randomized into 5 groups according to the body weight, namely normal group, model group, benzbromarone（20 mg·kg^-1·d^-1） group, high- and low-dosage chicory groups（10, 5 g·kg^-1·d^-1 respectively）. Except for the normal group, the quails in other groups were given high purine diet（ordinary forage mixed with 15 g·kg^-1·d^-1 of yeast powder） to induce hyperuricemia（HUA）model. And then we observed the changes of serum uric acid level and activities of uricopoiesis metabolic enzymes dynamically during the chicory treatment for 28 days. Results On the chicory administration day 7, 14, 21 and 28, serum uric acid level of quail was decreased obviously, and the activities of uricopoiesis metabolic enzymes of 5＇-nucleotidase（5＇-NT）, adenosine deaminase （ADA）,purine nucleoside phosphorylase（PNP）,guanine deaminase（GD）,xanthine oxidase（XO）were inhibited to various degrees in chicory groups（P〈0.05, P〈0.01 compared with the model group）. Conclusion Chicory has the effect on lowering serum uric acid level in quail hyperuricemia model, which may be associated with reducing the activities of 5＇-NT,ADA,PNP,GD,and XO.

关 键 词：高尿酸血症 菊苣 5'-核苷酸酶 嘌呤核苷磷酸化酶 腺苷脱氨酶 鸟嘌呤脱氨酶 黄嘌呤氧化酶 

分 类 号：R285.5[医药卫生—中药学]