表没食子儿茶素没食子酸酯对胆汁淤积性大鼠全基因表达谱的影响  

Effect of epigallocatechin gallate on gene expression of rats with cholestatic liver fibrosis

作　　者：喻冬柯[1,2] 张胜华[2] 邵荣光[2] 何红伟[2] 

机构地区：[1]四川省医学科学院四川省人民医院,成都610072 [2]中国医学科学院医药生物技术研究所,北京100050

出　　处：《中国新药杂志》2015年第2期143-147,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2012ZX09301002-001);国家自然科学基金(81170409);中国肝炎防治基金会王宝恩肝纤维化研究基金(20110026);四川省医学科学院四川省人民医院博士基金(30305030828)

摘　　要：目的:利用大鼠全基因组芯片检测表没食子儿茶素没食子酸酯(EGCG)对胆管结扎肝纤维化大鼠基因表达的影响并进行评价和研究。方法:使用胆管结扎的方法造胆汁淤积型的肝纤维化大鼠模型,并提取假手术组、模型组和给药组大鼠肝脏的总RNA,用大鼠全基因组芯片法分析其基因组表达谱的变化。结果:基因芯片结果显示,有626个基因同时在EGCG给药组与模型组发生变化,其中327个基因下调,299个基因上调。EGCG逆转了很多基因的表达变化,其中包括胆汁代谢通路、脂代谢、糖代谢等多种代谢相关通路、免疫相关通路等。此外,EGCG还影响多条肝纤维化相关的信号通路中相关基因的变化。结论:EGCG对胆汁淤积型肝纤维化大鼠模型的肝纤维化有抑制作用,该作用可能是通过调节多个肝纤维化相关的信号通路来发挥的。Objective： To investigate the role of epigallocatechin gallate（ EGCG） in the regulation of gene expression of liver fibrosis rats. Methods： Liver fibrosis rat model was induced by bile duct-ligation（ BDL）. The total RNA was isolated from the rats in sham-operation group,liver fibrosis model group,and EGCG group,and then screened for the differently expressed genes with gene chip technique. Results： The results of gene chip screening showed that there were 327 genes down-regulated and 299 genes up-regulated in the EGCG group compared to the liver fibrosis model group. These genes were related to bile acid biosynthesis,lipid metabolism,glucose metabolism pathway,immune-related pathways,and so on. EGCG also had effect on some liver fibrosis related pathway. Conclusion： EGCG has inhibition effect on the liver fibrosis of bile duct-ligated rat model,and the mechanism may be related to regulation of multiple signaling pathways.

关 键 词：表没食子儿茶素没食子酸酯 胆管结扎 肝纤维化 信号通路 基因表达谱 基因芯片 

分 类 号：R965.1[医药卫生—药理学] R975.5[医药卫生—药学]