钛种植体羟基磷灰石和神经生长因子复合涂层的制备及缓释性能研究  

Preparation of hydroxyapatite compound nerve growth factor complex coating and its controlled release on titanium surface

作　　者：张鹏 管红雨 钟小君 

机构地区：[1]佛山市口腔医院同济门诊部,广东佛山528000

出　　处：《广东牙病防治》2014年第5期238-240,共3页Journal of Dental Prevention and Treatment

基　　金：佛山市医学类科技攻关项目(201308091)

摘　　要：目的在钛种植体表面制备羟基磷灰石(hydroxyapatite,HA)和神经生长因子(nerve growth factor,NGF)复合涂层,为改善种植体骨感知能力,提高种植体与周围骨组织的结合提供思路。方法将钛种植体浸泡于模拟体液中获得HA钛种植体试件,将NGF与硫酸软骨素(chondroitinsulfate,CS)混合,冷冻干燥后形成NGF-CS纳米颗粒,将纳米颗粒与钛种植体试件共同浸泡于钙磷溶液中,获得HA-NGF钛种植体。种植体NGF的释放量采用酶联免疫吸附剂测定法测定,释放液中NGF活性采用PC12细胞存活法进行测定。结果成功制备出HA-NGF钛种植体试件,且试件有长达20 d的NGF缓慢释放,并且含NGF的试件组PC12细胞生存活性高于不含NGF的试件组,差异具有统计学意义(P<0.05)。结论钛种植体的HA-NGF复合涂层可以缓慢释放具有活性的NGF。Objective To form a complex coating of hydroxyapatite（ HA） compound nerve growth factor（ NGF） on the titanium（ Ti） surface,which provided a thought of modifying the ability of osseointegration and improving the osseointegration. Methods Ti was subjected to soaking in simulated body fluid（ SBF） to obtain the sample of HA-Ti. Meanwhile,the nanocomplex was fabricated by using an ionic interaction between NGF and chondroitinsulfate（ CS）. After that,the nanocomplex was precipitated on the HA-Ti surface,along with the addition of calcium / phosphate solution, the final product was formed as HA-NGF-Ti. The release of NGF was evaluated by ELISA. In addition,the viability of NGF was detected according to survival rate of PC12 cell. Results The sample of HA-NGF-Ti was fabricated successfully. The immobilized NGF was released from the sample HA-NGF-Ti in a moderate rate for 20 days. When PC12 cells were seeded on different Ti substrates,cell proliferation was faster in the HA-NGF-Ti group,as compared to other groups,there was significant difference（ P 〈 0. 05）. This study demonstrated that the delivery system of NGF was effective on the experiment group. Conclusion The activated NGF can obtain a controlled release from the HA-NGF complex coating on titanium surface.

关 键 词：神经生长因子 牙植入体 机械感受器 种植体 骨感知 

分 类 号：R783.1[医药卫生—口腔医学]