VEGF在EAE大鼠血清中的表达及意义  

The expression and significance of VEGF in the serum of EAE rats

作　　者：杨慧敏[1] 蹇顺海[1] 黄一凡[1] 李祖茂[1] 

机构地区：[1]川北医学院基础医学院病理学教研室,四川南充637000

出　　处：《川北医学院学报》2014年第5期442-445,共4页Journal of North Sichuan Medical College

基　　金：四川省教育厅自然科研基金(07ZB026)

摘　　要：目的:研究血管内皮生长因子(vascular endothelial growth factor,VEGF)及炎症相关细胞因子在实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)大鼠血清中的表达及探讨与疾病进展的关系。方法:雌性Wistar大鼠30只随机分为模型组20只及对照组10只,由豚鼠脊髓匀浆及百日咳菌液作为抗原免疫模型组大鼠制作EAE模型,于发病高峰时取得大鼠血清及脑脊髓标本,用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测血清VEGF、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)、干扰素-γ(IFN-γ)及髓鞘碱性蛋白(MBP)水平,组织学切片观察脑及脊髓病理变化。结果:(1)模型组大鼠全部发病,可见明显炎细胞浸润及髓鞘脱失改变;(2)VEGF在模型组大鼠血清中表达较对照组有明显增高(P<0.05),且VEGF水平的升高与动物模型发病临床评分呈显著正相关,与血清IFN-γ、MBP水平升高及IL-4水平降低亦相一致。结论:EAE血清VEGF水平与临床症状加重、促炎因子增高、抑炎因子降低及髓鞘破坏程度一致,提示VEGF可能通过与炎症因子协同作用参与了EAE的病变进展。Objective：To study the expression of VEGF and inflammation related cytokines in the serum of EAE rats and investigate the relationship with the progression of EAE. Methods：30 female Wistar rats were randomly divided into the model group（20）and the control group（10）. Model group had been immunized with guinea pig spinal cord homogenate and pertussis bacilli. Serum,brain and spinal cord specimens were obtained at peak of the disease. VEGF,IL-4,IL-10,IFN-γ and MBP levels in serum were measured by ELISA. Pathological changes in the brain and spinal cord were evaluated by histological sections. Results：（1）All model rats had symp-toms. Inflammatory cells infiltration and demyelination change were obvious in model rats. （2）The expression of VEGF in the serum of model group was significantly higher than the control group（P〈0. 05）. The increasing of VEGF was significantly correlated with clini-cal scores,and also consistent with the increasing of IFN-γ,MBP,the reducing of IL-4. Conclusion：VEGF levels in EAE serum were consistent with its clinical symptoms,the increasing of pro-inflammatory cytokines,the reducing of inhibition cytokines and the damage of myelin. VEGF may be synergistic with the inflammatory cytokines involved in the disease progression of EAE.

关 键 词：血管内皮生长因子 细胞因子 实验性自身免疫性脑脊髓炎 酶联免疫吸附法 

分 类 号：R361.1[医药卫生—病理学]