高脂血症对兔动脉管壁血流量及氧供的影响  

Effect of Hyperlipedemia on Rabbit Aortic Wall Blood Flow Volume and Oxygen Supply

作　　者：肖维刚[1,2] 张秋艳[2] 王宏涛 魏聪 贾振华[1,5] 

机构地区：[1]河北医科大学中西医结合教研室,河北省石家庄市050017 [2]河北以岭医药研究院 [3]河北省络病重点实验室 [4]国家中医药管理局心脑血管络病重点研究室,河北省石家庄市050035 [5]河北以岭医院心内科,河北省石家庄市050091

出　　处：《中国动脉硬化杂志》2014年第9期870-874,共5页Chinese Journal of Arteriosclerosis

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB518606)

摘　　要：目的观察高脂血症对兔动脉管壁微血管血流量及氧供的影响。方法将20只雄性新西兰兔随机分为正常对照组和高脂饮食组,每组10只,正常对照组饲喂普通饲料,高脂饮食组饲喂高脂饲料,实验4周时留取血流样本及主动脉组织样本,采用全自动生化分析仪测量其血脂四项水平,彩色微球技术测量其主动脉管壁微血管血流量,免疫组织化学染色观测其管壁微血管密度及缺氧诱导因子1α(HIF-1α)阳性表达,实时荧光定量逆转录聚合酶链反应(RT-qPCR)和Western blot测定动脉组织中HIF-1α的mRNA表达及蛋白水平。结果高脂饮食组血脂四项水平均显著高于正常对照组(P<0.01);高脂饮食组主动脉管壁微血管血流量较正常对照组明显增加[58.56±6.21μL/(min·g)比39.17±4.47μL/(min·g),P<0.01];高脂饮食组主动脉管壁微血管密度也显著高于正常对照组(5.1±0.4 n/mm2比2.7±0.7 n/mm2,P<0.01);而两组动脉组织中HIF-1α的mRNA表达及蛋白水平比较差异无统计学意义(P>0.05)。结论高脂血症增加了兔主动脉管壁微血管密度及微血管内的血流量,但并不影响动脉管壁的氧供。Aim To observe the effects of hyperlipidemia on the microvascular blood flow volume and oxygen supply of the rabbit aortic wall. Methods Twenty male New Zealand rabbits were randomly divided into normal control group or high-fat diet group,10 rabbits in each group,and were fed for 4 weeks. Blood and abdominal aorta samples were collected at the end of the study. The serum lipoprotein levels were measured by automatic biochemical analyzer,microvascular blood flow of the rabbit aortic wall was measured by color microspheres technology. Immunohistochemical staining was used to observe the microvessel density and the hypoxia condition in abdominal aorta wall. Hypoxia inducing factor-1α（HIF-1α） mRNA and protein expression of the arterial wall tissue were detected respectively by Western blot and Real-time fluorescent quantitative reverse transcription PCR（RT-qPCR）. Results Serum lipoprotein levels in highfat diet group were increased significantly compared to normal control group（all P 〈 0. 01）. Color microspheres test result showed the microvascular blood flow of the aortic wall in high-fat diet group was significantly higher than that in the normal control group [58. 56 ± 6. 21 μL /（min·g） vs. 39. 17 ± 4. 47 μL /（min·g）,P 〈 0. 01],and the wall microvessel density in high-fat diet group was also significantly higher than that in the normal control group（5. 1 ± 0. 4 n /mm2 vs.2. 7 ± 0. 7 n /mm2,P 〈 0. 01）,as HIF-1α expression also had no obvious changes in protein and mRNA levels（P 〉0. 05）. Conclusion Hyperlipidemia increases the microvessel density and microvascular blood flow volume of the rabbit aortic wall,but does not affect the oxygen supply.

关 键 词：高脂血症 动脉粥样硬化 微血管密度 血流量 缺氧诱导因子1Α 

分 类 号：R363[医药卫生—病理学]