2型糖尿病Kkay小鼠大血管病变模型的建立  

Establishment of Macroangiopathy Model of KKay Mice with Type 2 Diabetes Mellitus

作　　者：李海东[1] 庄晓明[2] 王泳[1] 

机构地区：[1]首都医科大学附属复兴医院高压氧科,北京市100038 [2]首都医科大学附属复兴医院内分泌科,北京市100038

出　　处：《中国动脉硬化杂志》2014年第11期1167-1169,共3页Chinese Journal of Arteriosclerosis

基　　金：北京市西城区科技计划项目(2008QKJ1024)

摘　　要：目的建立一种与人类发病过程类似的2型糖尿病大血管病变动物模型。方法选取24只12周龄2型糖尿病Kkay小鼠(尾尖取血测随机血糖≥11.1 mmol/L)作为模型组和12只C57BL/6J纯系小鼠作为正常对照组,两组小鼠都饲以高糖高脂饲料,模型组分别于16、20、24及28周各处死4只,模型组剩余小鼠和正常对照组小鼠于28周后处死,取腹主动脉于光镜下观察血管病变情况。每组实验动物在处死前测随机血糖。结果与正常对照组相比,模型组随机血糖均明显升高(P<0.01);模型组28周后出现大血管病变,表现为腹主动脉内皮细胞结构不清晰,质膜破裂,内弹力膜薄,内皮下层增厚,平滑肌细胞和成纤维细胞大量增殖,可见泡沫细胞。结论 2型糖尿病Kkay小鼠经高脂高糖饲养28周后可诱发大血管病变。Aim To establish animal models similar to human disease process of type 2 diabetes mellitus with macroangiopathy. Methods Twenty-four 12 week old Kkay mice with type 2 diabetes mellitus were selected（ random blood sugar≥11. 1 mmol / L） as model group and twelve C57 BL /6J mice as normal control group,experimental animals of the two groups were fed with high sugar and high fat diet,four mice were killed in the model group at the 16 th,20 th,24 th and 28 th week. Remaining mice in the model group and normal control group were killed after 28 weeks,and abdominal aorta were observed under light vascular lesions. Results Compared with normal control group,random blood sugar in model group were significantly higher（ P 〈 0. 01）. Macroangiopathy in the model group was found after 28 weeks,it’s shown as that abdominal aortic endothelial cell structure was not clear,plasma membrane rupture,internal elastic membrane was thin,subendothelial layer thickening,smooth muscle cells and fibroblasts proliferation in great quantities,foam cells cloud be found. Conclusion Kkay mice with type 2 diabetes mellitus by feeding high fat and high sugar after 28 weeks may induce macroangiopathy.

关 键 词：2型糖尿病 大血管病变 KKAY小鼠 

分 类 号：R36[医药卫生—病理学]