二十五味珊瑚丸对D-半乳糖衰老大鼠神经元特异性烯醇化酶表达的影响  

Effect of 25 Odors Coral Pills on Pyramidal Cell and the Expression of NSE in Hippocampus of D-galactose Induced Brain Aging Rats

作　　者：杨春[1,2] 李鹏[3] 罗远带[4] 杜以宽[3] 黄福开[4] 甄丽芳[4] 胡贤达 杨勤劳[1] 原林[3,2] 

机构地区：[1]深圳大学光电工程学院光电子器件与系统(教育部/广东省)重点实验室,深圳518000 [2]深圳大学医学院,深圳518060 [3]南方医科大学,广州510515 [4]中国藏学研究中心北京藏医院,北京100029

出　　处：《天然产物研究与开发》2015年第1期45-49,共5页Natural Product Research and Development

基　　金：国家重大财政专项:藏医心脑血管临床研究与药物研发(1981020421);省产业技术研究与开发资金项目(2011B010500004)

摘　　要：探讨二十五味珊瑚丸(25 Odors Coral Pills)对D-半乳糖(D-gal)衰老大鼠海马锥体细胞形态和神经元特异性烯醇化酶(NSE)表达的影响。采用颈背部皮下注射D-gal模拟衰老大鼠模型。治疗组于造模第6周,给予25 Odors Coral Pills灌胃2周,之后取各组脑组织行相关检测。与模型组比较,25 Odors Coral Pills组大鼠海马CA1、CA3区锥体细胞层细胞丢失较少,细胞排列较紧密、整齐;DG区NSE阳性的细胞减少,胞体较小,突起较细;海马NSE表达下降,有统计学差异(P<0.05)。D-gal衰老模型鼠海马锥体细胞衰老变性且NSE的表达增多;25 Odors Coral Pills可抑制D-gal衰老鼠海马锥体细胞的衰老变性和NSE的表达。The D-galactose induced brain aging model was used to study the effect of 25 odors coral pills on hippocampus. The morphology of pyramidal cells and the expression of neuron-specific enolase（NSE） in hippocampus were determined in each experimental group. Hippocampal neuronal degeneration on CA1,CA3,DG region was observed by hematoxylin-eosin staining. The expression of NSE was explored by immunohistochemistry,Western blotting and densitometric analysis. The results showed that the degeneration of hippocampal pyramidal cells in 25 odors coral pills group was significantly improved,and the number of cells positive for NSE and the expression of NSE in the hippocampus were significantly reduced. These results indicated that 25 odors coral pills can inhibit hippocampal neuronal degeneration and the expression of NSE protein in the D-galactose induced brain aging rats.

关 键 词：二十五味珊瑚丸 神经元特异性烯醇化酶 D-半乳糖 衰老 锥体细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]