UPLC-MRM-MS法测定头花蓼药材中7个指标成分  

Determination of Seven Components in Polygonum capitatum by UPLC-MRM-MS

作　　者：刘跃[1] 胡杰[1] 谢玉敏[1] 黄勇[1] 廖尚高[2] 郑林[1] 

机构地区：[1]贵阳医学院药学院贵州省药物制剂重点实验室 [2]贵阳医学院民族药与中药开发应用教育部工程研究中心,贵阳550004

出　　处：《天然产物研究与开发》2015年第1期73-76,88,共5页Natural Product Research and Development

基　　金：国家自然科学基金项目(81260688;81160516);国家科技支撑计划(2013BAI11B01);贵州省国际科技合作计划(2012-7040)

摘　　要：建立头花蓼药材中没食子酸、原儿茶酸、陆地棉苷、槲皮苷、儿茶素、槲皮素-3-O-（2″-没食子酰基）-鼠李糖苷及槲皮素的UPLC-MRM-MS检测方法。色谱采用Waters BEH C18（2.1 mm×50 mm,1.7μm）色谱柱,以0.1%甲酸乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,质谱采用多反应离子监测（MRM）检测。没食子酸、原儿茶酸、陆地棉苷、槲皮苷、儿茶素、槲皮素-3-O-（2″-没食子酰基）-鼠李糖苷及槲皮素分别在0.136~32.965、0.031~7.481、0.022~5.300、0.076~18.433、0.085~20.737、0.017~4.147、0.128~31.029μg/m L浓度范围线性关系良好,平均回收率为91.23%~105.33%,RSD〈3%,精密度、重复性和稳定性良好。此方法操作简便,快速,灵敏度高,可用于头花蓼药材中主要活性成分的含量测定,并为头花蓼药材质量控制提供了新方法。To establish a UPLC-MRM-MS method for the determination of gallic acid,protocatechuic acid,que-3-O-β-DGlc,quercitrin,catechin,que-3-O-（2″-O-galloyl）-β-D-Glc and quercetin in Polygonum capitatum. The analysis was achieved by BEH C18 column with a mobile phase composed of 0. 1% formic acid in acetonitrile and 0. 1% formic acid in water using gradient elution. A TQD tandem mass spectrometry was used as detector and operated by multiple reaction monitoring（MRM） mode. Good linearity was achieved for these components in the range of 0. 136-32. 965,0. 031-7.481,0. 022-5. 300,0. 076-18. 433,0. 085-20. 737,0. 017-4. 147,0. 128-31. 029 μg/m L,respectively. The average recoveries were 91. 23%-105. 33%,with RSD less than 3%. Precision,repeatability and stability were good. The developed method was simple,rapid,sensitive and suitable for the determination of the main active constituents in P. capitatum. It provided a new method for the quality control of P. capitatum.

关 键 词：头花蓼 UPLC-MRM-MS 没食子酸 原儿茶酸 槲皮素 含量测定 

分 类 号：R927.2[医药卫生—药学]