黑果枸杞对X射线辐射小鼠的保护作用研究  

Protective Effects of Lycium ruthenicum Murr on X-Radiation Injured Mice

作　　者：段雅彬 姚星辰[1] 王财[2] 陈湘宏[1] 张娟玲[1] 李向阳[1] 

机构地区：[1]青海大学医学院 [2]青海大学附属医院,青海西宁810001

出　　处：《天然产物研究与开发》2015年第1期148-152,共5页Natural Product Research and Development

基　　金：青海省自然科学基金项目(2013-Z-909);教育部新世纪优秀人才支持计划(NCET-10-0917)

摘　　要：探讨黑果枸杞水提取液对辐射损伤小鼠的保护作用。除正常对照组外各组小鼠接受5Gy的X射线一次性全身均匀照射。检查各组小鼠外周血中红细胞数(RBC)、白细胞数(WBC)、血小板数(PLT)、血红蛋白数(HGB),计算胸腺和脾脏指数,测定抗氧化酶活力、DNA和Caspase-3、Caspase-6的含量。黑果枸杞水提取液对辐射后小鼠的血象有明显回升,SOD活力明显增强,DNA含量增多,CAT、GSH-PX、T-AOC活力下降,Caspase-3和Caspase-6的含量降低,脏器指数无统计学意义。研究结果显示,黑果枸杞水提取液对X射线所致小鼠辐射损伤有一定保护作用。To study the protective effects of Lycium ruthenicum Murr extract against radiation-induced damage on mice.Except the normal control group,mice of other groups were received with 5 Gy of X-radiation. Red blood cell count（RBC）,white blood cell count（WBC）,platelet count（PLT）,hemoglobin count（HGB）,thymus index and spleen index,activity of various antioxidant enzymes,DNA and caspase-3,caspase-6 content were observed at 7 d after radiation.The results showed that L. ruthenicum extract significantly increased quantity of hemogram,activity of SOD,content of DNA in bone marrow. The activity of CAT,GSH-PX,T-AOC and the content of caspase-3,caspase-6 significantly reduced. In addition,the spleen index,thymus index had no statistical significance in our study. These results indicated that L. ruthenicum had protective effects on mice radiation damage.

关 键 词：黑果枸杞 抗辐射 清除自由基 抗氧化作用 

分 类 号：R965.1[医药卫生—药理学]