丹莲肝康颗粒中药材的提取工艺研究  

Study on the Extraction Process of Chinese Medicinal Materials in Danlian Gankang Granules

作　　者：李智勇 王洛临 施之琪 徐文杰 陈雪婷 

机构地区：[1]广东省中医药工程技术研究院,广州510095

出　　处：《中国药房》2015年第4期522-525,共4页China Pharmacy

摘　　要：目的:优选丹莲肝康颗粒中药材的提取工艺。方法:以丹参酮ⅡA提取率和干膏得率为指标,采用均匀设计法优选丹参和紫草醇提工艺中的乙醇体积分数、乙醇用量、浸泡时间、提取时间、提取次数;以野黄芩苷和丹酚酸B的提取率及干膏得率为指标,采用正交设计试验法对水提工艺中的加水量、提取时间、提取次数进行优化,对最优处方工艺进行验证。结果:最佳醇提工艺为丹参、紫草加9.6倍95%乙醇,浸泡3 h,提取2次,每次20 min;3次验证试验显示,丹参酮ⅡA提取率平均值为44.73%,干膏得率平均值为25.55%。药渣和其余药材加水提取3次,第1次加水12倍量水,第2、3次分别加水10倍量,每次提取1 h;3次验证试验丹酚酸B提取率平均值为47.67%,野黄芩苷提取率平均值为71.67%,干膏得率平均值为24.63%。结论:优选的提取工艺稳定可行,为丹莲肝康颗粒的制备提供了科学依据。OBJECTIVE： To optimize the extraction process of Chinese medicinal materials in Danlian gankang granules. METHODS： With the index of extraction rate of Tanshinone ⅡA and dry extract yielding rate, uniform design was conducted to optimize the alcohol extraction process of Salvia and Arnebiae Radix in respects of alcohol concentration, the amount of ethanol, soaking time, extraction time and extraction times. With the index of extraction rate and dry extract yielding rate of scutellarin and Sal B, orthogonal experiment method was conducted for the optimization of water extraction amount, extraction time and extrac- tion times and verification of optimal formulation process. RESULTS ： The optimum alcohol extraction process was as follows, Sal- via and Arnebiae Radix were added 9.6 times of 95% ethanol with 3 h soaking time and twice of extraction time each for 20 min. The average extraction rate of 3 Tanshinon Ⅱ A verification tests was 44.73% ; dry extract yielding rate was 25.55%. And the residu- al dregs with other remaining herbs were extracted three times with water, 12 times the amount of water was added for the first time, 10 times for the second and third time, with 1 h extract each time. In three verification tests, Sal B extraction rates were with an average of 47.67% ; scutellarin extraction rates were with an average of 71.67% ; dry extract yielding rate were with an av- erage of 24.63%. CONCLUSIONS： The optimized extraction process is stable and feasible, It provides a scientific reference for the preparation of Danlian gankang granules.

关 键 词：丹莲肝康颗粒 提取工艺 丹参酮ⅡA 丹酚酸B 野黄芩苷 

分 类 号：R914[医药卫生—药物化学] R284.2[医药卫生—药学]