马铃薯‘H-027'与‘陇薯6号'和‘陇薯7号'杂种优良单株的SSR分析  

SSR analysis on potato superior hybrid individual from 'H-027' × 'Longshu No.6'And'H-027' × 'Longshu No.7'

作　　者：于卓[1] 郝治满 鞠天华 于肖夏[1] 温贺[1] 崔阔澍[1] 姜超[1] 王丹[1] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]赤峰天泽生物科技有限公司,内蒙古赤峰025457

出　　处：《中国园艺文摘》2015年第1期8-11,共4页Chinese Horticulture Abstracts

基　　金：国家科技支撑计划项目(2012BAD02B05);内蒙古自然科学基金重大项目(2013ZD03);内蒙古马铃薯产业发展重大技术集成创新与示范项目(20131706)资助

摘　　要：为了明确马铃薯杂种F_1单株的真实性,利用SSR分子标记技术对‘H-027'ב陇薯6号'和‘H-027'ב陇薯7号'这2个马铃薯杂交组合F_1的优良单株进行鉴定。试验从87对SSR引物中筛选出3对适宜引物S7,S1 70和S174;用引物S7和S170从马铃薯‘H-027'ב陇薯6号'的杂种F_1的30个优良单株鉴定出25个真实杂交种单株;用引物S170和S174鉴定出‘H-027'ב陇薯7号'杂种F_1的14个优良单株均为真实杂交种。In order to determine the authenticity of F1 superior individuals of potato hybrid,the SSR molecular marker technology was used to identify the F1 superior individuals of two hybrid combinations ＇H-027＇ × ＇longshu No.6＇ and ＇H-027＇ × ＇longshu No.7＇.Three suitable primer pairs S7,S170 and S174 were selected from 87 SSR primer pairs.Using the SSR primers S7 and S170,a total of 25 true hybrid plants were identified from 30 superior individuals of the hybrid combination ＇ H-027＇ × ＇ longshu 6＇.Using the primers S170 and S174,all the 14 superior individuals of the hybrid combination ＇H-027＇ × ＇longshu 7＇ were identified as true hybrid plants.

关 键 词：马铃薯 杂种F_1 优良单株 SSR分析 

分 类 号：S792.95[农业科学—林木遗传育种]