柚皮中抑菌物提取工艺优化研究  

Study on the Optimal Extraction Technology of Antimicrobial Substance from Pomelo Peels

作　　者：田广文[1] 李鹏飞[1] 杜丽华[1] 张晶[1] 张学伟[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《安徽农业科学》2015年第5期259-262,265,共5页Journal of Anhui Agricultural Sciences

基　　金：西北农林科技大学校重点创新实验项目(1210712162)

摘　　要：[目的]优化柚皮提取类黄酮、类柠檬苦素的工艺条件。[方法]以柚皮为原料,采用"乙醇水浴法"提取类黄酮,采用"二氯甲烷回流法"提取类柠檬苦素。[结果]提取类黄酮的最佳工艺为乙醇体积分数60%、料液比1∶25 g/ml、提取温度70℃、提取时间1.5 h,提取次数2次,类黄酮3次平均提取率为1.211%;提取类柠檬苦素的最佳工艺为料液比1∶15 g/ml,温度55℃,提取2.5 h,提取次数2次,类柠檬苦素3次平均提取率为0.561%。[结论]所得的多元二次回归方程预测模型与实际情况拟合较好,可用于指导柚皮抑菌物的提取分离。[ Objective ] The paper aims to set up an optimal method for extraction technology of flavonoids and limonolds from pomelo peels. [ Method ] Using ＂ethanol water bath method＂ extracts flavonoids; using ＂dlehloromethane refluxing method＂ extracts limonoids. [ Result ] The optimal extraction conditions of flavonoids were ethanol concentration 60% ,solid-liquid ratio 1：25 g/ml,extraeting temperature 70 ~C ,extracting time 1.5 h and extraction 2 times. In these conditions,the average of three actual verification extraction yield of flavonoids was 1.211%. The optimum extraction condition of limonoids were solid-liquid ratio 1 ： 15 g/ml, extracting temperature 55 ℃, extracting time 2.5 h and extraction 2 times. In these conditions,the average of three actual verification extraction yield of limonoids was 0.561%. [ Conclusion ] The resulting multivariate quadratic regression equation prediction model fit well with the actual situation, can be used to guide the extraction and separation of antimicrobial substance from pomelo peels.

关 键 词：柚皮 类黄酮 类柠檬苦素 均匀设计 响应面分析 

分 类 号：S666.3[农业科学—果树学] TS255.1[农业科学—园艺学]