铁离子致大鼠实验性脑室出血后急性脑损伤的初步研究  

Iron ion-induced acute brain injury in rats with intraventricular hemorrhage

作　　者：张建波[1] 唐俊[2] 杨力铭[2] 陈前伟 郭靖[2] 陈志[2] 朱刚[2] 

机构地区：[1]广州军区联勤部门诊部,广州510000 [2]第三军医大学西南医院神经外科,全军神经外科研究所,全军神经系统疾病微创诊治专科中心,全军神经创伤防治重点实验室,重庆400038

出　　处：《第三军医大学学报》2015年第1期5-10,共6页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81271281;81070929);国家重点基础研究发展计划(973计划;2014CB541606)~~

摘　　要：目的观察大鼠脑室内注入铁离子后早期脑损伤的基本病理特点,探讨铁离子在大鼠脑室出血后急性期脑损伤中的作用。方法通过立体定向侧脑室注射法建立脑室出血后铁损伤模型,侧脑室注射二氯化铁或生理盐水,采用干湿质量法测定损伤后24 h脑组织含水量变化,伊文思蓝染色法观察血脑屏障损伤,Fluoro-Jade C(FJC)、TUNEL染色及透射电镜观察海马神经元变性、凋亡情况,采用透射电镜观察脑室壁纤毛损伤,并于损伤后7 d采用整体脑室剥离法观察大体标本脑室壁含铁血黄素沉积。结果铁损伤组在24 h时大脑半球含水量较对照组明显升高(P<0.05)、血脑屏障破坏、海马神经元大量变性、凋亡,脑室室管膜纤毛及微绒毛大量脱落;7 d时侧脑室壁大量含铁血黄素沉积。结论铁离子脑室内沉积可导致脑水肿、血脑屏障损伤、脑室壁损伤等急性脑损伤效应,可能在脑室出血后急性期脑损伤中起重要作用。Objective To investigate the pathological features of intraventricular injection of iron ion in rats with intraventricular hemorrhage （IVH） Methods Male Sprague-Dawley rats were given and explore the role of iron in acute brain injury after IVH. an injection of 100 μL either normal saline （Control） or ferrous chloride （FeC12, the iron group） into the right lateral ventricle, and were euthanized at different time points. Brain edema was evaluated by wet-dry weighting in 24 h after injury. Evans blue （EB） staining was used to investigate blood brain barrier （BBB） injury. Terminal-deoxynucleotidyl transferase Mediated nick end labeling （TUNEL）, Fluoro-Jade C （FJC） staining and transmission electron microscopy （TEM） were carried out to test the degeneration and apoptosis of hippocampus neurons and observe the damage in ventricular cilia. Hemosiderin deposition was investigated by stripping the whole cerebral ventricles in 7 d after injury. Results The iron group had significantly increased brain water content （P 〈 0.05 ） , damaged BBB, large amount of hippocampal neurons degenerated and apoptotic, and a great deal of cilia and microvilli shedding from ventricular ependyma in 24 h after the injury, and lot of hemosiderin deposition on the lateral ventricular wall in 7 d as well when compared to control group. Conclusion Intraventricular deposition of iron causes brain edema. BBB damaze, injury of ventricular wall and other svmotoms of acute brain iniurv, suggesting that iron may play an important role in the acute injury after IVH.

关 键 词：二氯化铁 脑水肿 血脑屏障 大鼠 

分 类 号：R363.13[医药卫生—病理学] R742[医药卫生—基础医学]