TNF-α和HSP70-2基因多态性与急性胰腺炎的相关性研究  

Polymorphisms of TNF-α gene and HSP70-2 gene in patients with acute pancreatitis

作　　者：胡家平[1] 庄建新[1] 李勇[1] 余永欢[1] 胡庆红[1] 赖银英[1] 吴安涛[1] 

机构地区：[1]南昌大学第一附属医院普外科五病区,330006

出　　处：《天津医药》2015年第1期57-60,共4页Tianjin Medical Journal

基　　金：江西省科技厅支撑计划

摘　　要：目的探讨肿瘤坏死因子(TNF)-α和热休克蛋白(HSP)70-2基因多态性与急性胰腺炎(AP)的相关性。方法运用聚合酶链式反应-限制性片段长度多态性(PCR-RLFP)检测72例AP患者(AP组)和71例正常人(对照组)的TNF-α及HSP70-2基因多态性。结果 AP组与对照组的TNF-α基因型和等位基因比例间差异无统计学意义;AP组中,重型胰腺炎(SAP)和轻型胰腺炎(MAP)的TNF-α基因型和等位基因间差异无统计学意义。TNF-α和HSP70-2基因AA型与GA+GG型患者的白细胞、C反应蛋白(CRP)、淀粉酶、三酰甘油、血糖差异均无统计学意义;AP组的HSP70-2基因GA+GG型患者比例高于对照组(69.4%vs 49.3%),AP组携带的G等位基因比例高于对照组(46.5%vs 31.7%),差异均有统计学意义。AP组中,SAP的GA+GG型比例高于MAP(81.0%vs 53.3%),差异有统计学意义;而G等位基因在SAP和MAP间无明显差异(P>0.05)。结论 TNF-α基因多态性与AP不相关,HSP70-2基因多态性与AP相关,携带G等位基因发生重症AP的可能性增大,可能是重症AP的易感因素之一。Objective To investigate the association of tumor necrosis factor （TNF）-α, heat shock protein （HSP）70-2 gene polymorphisms and susceptibility of acute pancreatitis（AP）. Methods Using case-control method,The gene polymor？phism of TNF-α and HSP70-2 was detected by PCR-RLFP in 72 patients with AP and 71 healthy controls. Results There were no significant differences in proportion of TNF-αgenotype and alleles between AP and control groups （P〉0.05）. There were no significant differences in TNF-αgenotype and alleles between severe acute pancreatitis （SAP） and light acute pancreatitis （MAP） of AP group （P〉0.05）. There were no significant differences in white blood cell count, C-reactive pro？tein （CRP）, amylase, three acyl glycerin and glucose between TNF-a and HSP70-2 gene of AA type and GA＋GG type pa？tients （P〉0.05）. The HSP70-2 genotype GA＋GG proportion was significantly higher in AP group than that of control group （69.4%vs 49.3%）. The ratio of patients with G allele was significantly higher in AP group than that of control group（46.5%vs 31.7%）. The ratio of patients with GA＋GG type AP was significantly higher in SAP patients than that of MAP patients of AP group（81.0% vs 53.3%）. There was no significant difference in G allele between SAP and MAP patients （P〉0.05）. Conclusion TNF-α polymorphisms is not associated with acute pancreatitis. There is an association between HSP70-2 polymorphisms and acute pancreatitis. Carrying the G allele increases the possibility of a severe acute pancreatitis ,which is one of the genetic susceptibility factors of severe acute pancreatitis.

关 键 词：肿瘤坏死因子Α HSP70热休克蛋白质类 胰腺炎 基因多态性 HSP70—2 多态性 限制性片断长度 聚合酶链反应 

分 类 号：R576[医药卫生—消化系统]