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机构地区:[1]南华大学附属第一医院皮肤科,湖南衡阳421001 [2]中南大学湘雅三医院皮肤科,湖南长沙410013
出 处:《中国皮肤性病学杂志》2015年第1期5-8,共4页The Chinese Journal of Dermatovenereology
摘 要:目的通过观察补骨脂注射液联合窄谱中波紫外线(NB-UVB)对人黑素瘤A375细胞的增殖、黑素的合成以及酪氨酸酶活性的影响,探讨两者协同治疗白癜风的可能机制。方法选择一定浓度的补骨脂注射液(稀释后浓度为0.5%)联合一定剂量的NB-UVB(22m J/cm2)共同作用于人黑素瘤A375细胞后,通过MTT法观察其对人黑素瘤A375细胞细胞增殖的影响,Na OH裂解法测定黑素的含量,多巴氧化法测定酪氨酸酶活性的变化。结果补骨脂注射液+NB-UVB组中A375细胞培养至48h,72h后检测到的细胞增殖情况与单一补骨脂注射液组、NB-UVB组及空白组相比较,差异有统计学意义(P<0.05)。补骨脂注射液+NB-UVB组均显示可上调细胞内酪氨酸酶的活性及黑素的生成量,且与各实验干预组及空白组比较,差异有统计学意义(P<0.05)。结论一定浓度的补骨脂注射液(稀释后浓度为0.5%)联合一定剂量的NB-UVB(22m J/cm2)可以协同促进人黑素瘤A375细胞的增殖,上调细胞酪氨酸酶活性,从而达到促进黑素合成的效果。Objective To discuss possible mechanism of NB-UVB combined with psoralen injection for the synergistic treatment of vitiligo through studying the cell proliferation, melanogenesis and tyrosinase activity of Human melanoma A375 cells treated by NB-UVB combined with psoralen injection. Methods Human melanoma A375 cells treated with a certain concentration gradient of psoralen injection( the concentration was 0.5% by after being appropriately diluted)and a certain intensity of NB-UVB (22mJ/cm2) were used to detectet the growth by MTT, the content of melanin with the law of spallation of NaOH, and the changes of tyrosinase activity with dioxypenylalanine oxidation. Results Compared with the psoralen injection group, NB-UVB group and the blank group, cell proliferation was increased obviously at 48h and 72h( P 〈 0.05 ) in the psoralea injection + NB-UVB group. Compared with the blank group and the intervention groups, the melanin content and tyrosinase activity were both up-regulated in the psoralen injection + NB-UVB group, the difference showed significant (P 〈 0.05 ). Conclusion A certain concentration gradient of psoralen injection ( the concentration was 0.5% by after being appropriately diluted)and a certain intensity of NB-UVB(22mJ/cm2) can promote together A375 melanocyte proliferation, and increase cell tyrosinase activity ,which leads to achieve the effect of promoting melanin synthesis.
关 键 词:人黑素瘤A375细胞 补骨脂注射液 NB—UVB 酪氨酸酶活性 黑素含量
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