雌二醇及中波紫外线在豚鼠皮肤色素沉着形成中的作用及其机制  

The effect and mechanism of estradiol and UVB radiation on pigmentation of guinea-pig skin

作　　者：周颖丽[1] 宋秀祖[1] 洪为松[1] 相文忠[1] 

机构地区：[1]安徽医科大学杭州临床学院(杭州市第三人民医院)皮肤性病科,杭州310009

出　　处：《安徽医科大学学报》2015年第1期49-53,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81000697);杭州市医学重点专科专病项目(编号:20130733Q24)

摘　　要：目的研究雌二醇和中波紫外线(UVB)辐射对豚鼠背部棕色皮肤中黑素代谢的影响,探讨雌激素及UVB在皮肤色素沉着中的作用。方法选取健康花色豚鼠,设立溶媒对照组、UVB照射组、雌二醇处理组和雌二醇预处理后UVB照射组(雌二醇+UVB组);雌二醇浓度设为3.67×10-5、3.67×10-4、3.67×10-3mol/L;UVB照射剂量统一为500mJ/cm2。采用皮肤在体共聚焦激光扫描显微镜(简称皮肤CT)以及透射电子显微镜等观察雌二醇和UVB对黑素小体合成及降解的影响。结果 1一定浓度雌二醇可加深豚鼠皮肤色素沉着,且随着雌二醇浓度的递增,沉着程度逐渐加深(P<0.05)。2 UVB照射组豚鼠皮肤色素沉着较未照射组明显增加(P<0.05)。3 UVB照射后豚鼠表皮角质形成细胞中自噬体增加,而雌二醇处理后自噬体减少。结论雌二醇及UVB照射均可促进豚鼠皮肤色素沉着,其机制可能与促进黑素小体合成有关,黑素小体的降解异常也参与了豚鼠皮肤色素沉着的形成。Objective To investigate the influence of estradiol and ultraviolet B （ UVB） radiation on melanin me-tabolism of guinea-pig’ s brown dorsal skin, and explore the relationship between the estrogen and ultraviolet in skin pigmentary disorder. Methods Healthy suit guinea-pigs were selected in this study. The guinea-pigs skin was di-vided into solvent control group, UVB irradiation group, estradiol treatment group and UVB irradiation after estradi-ol pertreatment group （ estradiol ＋ UVB group） . The concentrations of estradiol were set to 3. 67 × 10^ -5 ,3. 67 × 10^ -4 ,3. 67 × 10^ -3 mol/L. The UVB exposure dose was unified as 500 mJ/cm^2 . In vivo confocal laser scanning mi-croscopy （ referred to as skin CT） and transmission electron microscopy were used to observe the effects of estradiol and UVB on melanin metabolism and degradation. Results ①The pigmentation of guinea-pig skin was deepened after a certain concentration of estradiol treatment, increased gradually with the increasing of estradiol concentration （P〈0. 05）. ② The pigmentation of guinea-pig skin was also deepened significantly in UVB irradiation group, compared with non-UVB irradiation group（P〈0. 05）. ③The autophagy of epidermal keratinocytes of Guinea pigs increased with UVB irradiation, but decreased with estradiol treatment. Conclusion Estradiol and UVB irradiation can promote pigmentation of guinea-pig skin and the mechanism may be related to the promotion of melanosomes synthesis. The degradation of abnormal melanosomes may also be involved in the formation of pigmentation.

关 键 词：雌二醇 色素沉着 黑素小体 中波紫外线 

分 类 号：R364.23[医药卫生—病理学]