玻璃体腔注射神经生长因子安全性研究及视网膜组织分布  

Safety of intravitreal injection of nerve growth factor and its distribution in retina

作　　者：刘丹宁[1,2] 樊继山[3] 阴正勤[2] 

机构地区：[1]重庆医科大学附属第二医院眼科,400010 [2]第三军医大学西南医院眼科,重庆400038 [3]重庆医科大学儿童医院药学部,400014

出　　处：《重庆医学》2015年第3期289-292,共4页Chongqing medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB512203);重庆市卫生局医学科研计划项目(2013-2-047)

摘　　要：目的观察兔玻璃体腔注射鼠神经生长因子(mNGF)的安全性及其在视网膜组织的分布。方法兔玻璃体腔注射临床剂型mNGF 30μg/100μL后1、7、30d进行行为学观察、裂隙灯眼前节检查、眼底检查、眼部B超及组织病理学检查,确定其使用安全性。玻璃体腔注射该剂量标记后的125I-NGF 15、30min,1、3、6、8、12、24、48h,观察视网膜药物峰浓度及达峰时间。结果兔眼玻璃体腔注射mNGF后,眼前节及眼底均未见明显损伤及炎性反应,眼部B超及组织HE染色未见明显眼组织结构异常。视网膜药物含量可于注射后3h达峰值(118.32±18.74)%ID/g,为眼部各组织中最高。结论玻璃体腔注射mNGF是安全的,玻璃体腔注射mNGF能够迅速在视网膜组织中聚集,达到较高药物浓度。Objective To observe safety of intravitreal injection of mouse nerve growth factor and its distribution in retina in rabbits .Methods The behavioral observation ,slit lamp examination ,fundus examination ,eye B ultrasonic and histopathological ex‐amination were carried out on 1 ,7 and 30 d after intravitreal injection 30 μg/100 μL mNGF to determine the safety in eye .The dis‐tribution and peak time in retina were investigated at 15 ,30 min ,1 ,3 ,6 ,8 ,12 ,24 ,48 h after intravitreal injection 125 I‐NGF 30 μg/100 μL .Results No abnormal changes were found in their cornea ,lens ,vitreous body and retina after mNGF intravitreal injection . And the each layer of retinal cells layout were regular according to the result of morphological observation on 30 days after treat‐ment .The peak concentration of mNGF in retina and the highest in whole eye was （118 .32 ± 18 .74）% ID/g and the peak time was at 3 hour after injection .Conclusion It is safe for intravitreal injection of mNGF and mNGF could gather in retina quickly after in‐travitreal injection .

关 键 词：神经生长因子 玻璃体 视网膜 安全性 

分 类 号：R774.1[医药卫生—眼科]