胆管Cajal间质细胞与药物性肝内胆汁淤积的相关性研究  

Study of association between interstitial Cells of cajal in bile duct and drug-induced intrahepatic cholestasis

作　　者：蔡佳[1] 章述军[1] 秦波[1] 

机构地区：[1]重庆医科大学附属第一医院感染科,400016

出　　处：《重庆医学》2015年第3期306-308,311,共4页Chongqing medicine

基　　金：重庆市卫生局课题(2010-2-006)

摘　　要：目的通过研究大黄素对药物性肝内胆汁淤积症模型相关的生化指标及Cajal间质细胞(ICC)的影响,以期发现胆管ICC在肝内胆汁淤积中的作用及大黄素对ICC及肝内胆汁淤积的影响。方法 15只雄性SD大鼠采用简单随机法均分为3组,即对照组、药物性肝内胆汁淤积模型组及大黄素干预组(n=5)。建立大鼠淤胆型肝炎及大黄素干预的模型。使用反转录PCR(RT-PCR)及免疫组织化学法分别检测模型组、大黄素组及对照组间肝功能生化指标、干细胞因子受体c-kit基因及蛋白表达水平。结果模型组肝功能生化指标水平与对照组相比明显升高(P<0.05),大黄素组上述指标水平较对照组也有明显升高,但较模型组低,其间差异有统计学意义(P<0.05)。对照组、模型组及大黄素组位于548bp处的条带均有表达。模型组c-kit mRNA表达量明显低于干预组及对照组(P<0.05)。大黄素组低于对照组,组间差异无统计学意义(P>0.05)。免疫组织化学结果显示c-kit模型组中表达量明显低于对照组及大黄素组(P<0.05)。结论胆管ICC与药物性肝内胆汁淤积形成过程可能存在密切关系,肝内胆汁淤积的形成与胆管ICC减少有关,而大黄素对肝内胆汁淤积的治疗作用可能与其所致的胆管ICC数量相对增多或对胆管ICC的保护作用有关。Objective To study the effect of emodin on biochemical indicators of drug‐induced intrahepatic cholestasis model and the interstitial cells of cajal （ICC） in bile duct and to explore the role of ICC and emodin in intrahepatic cholestasis .Methods Fif‐teen rats were randomly divided into drug‐induced intrahepatic cholestasis group ,emodin intervention group and control group（n=5） .Rat cholestatic hepatitis model and emodin intervention model were established .RT‐PCR and immunohistochemistry were used to detect liver function ,c‐kit mRNA and protein expression levels in drug‐induced intrahepatic cholestasis group ,emodin interven‐tion group and control group .Results The degree of liver dysfunction and bilirubin level in drug‐induced intrahepatic cholestasis group were significantly higher than those in control group（P0 .05） .Immunohistochemistry results indi‐cated that expression of c‐kit in drug‐induced intrahepatic cholestasis group was significantly lower than those in control group and emodin intervention group（P〈0 .05） .Conclusion There may be close relationship between the forming process of drug‐induced in‐trahepatic cholestasis and decrease of ICC in bile duct .The therapeutic effect of emodin on intrahepatic cholestasis may be related with the number of ICC in bile duct or the positive effect on ICC.

关 键 词：肝内胆汁淤积 大黄素 C-KIT 胆管ICC 

分 类 号：R575[医药卫生—消化系统]