胃癌中葡萄糖调节蛋白78的表达及其临床病理学意义  

The Expression and Clinical Pathological Significances of GRP78 in Human Gastric Cancer Tissues

作　　者：贺正希[1] 唐慧岚 陈景飞[1] 陈文琳[1] 蒋炜峥 谢远杰[2] 

机构地区：[1]南华大学医学院 [2]南华大学医学院组织胚胎学教研室

出　　处：《中南医学科学杂志》2015年第1期21-25,共5页Medical Science Journal of Central South China

基　　金：2012年湖南省大学生研究性学习和创新性实验计划项目;2012年地方高校国家级大学生创新创业训练计划项目(201210555017)资助

摘　　要：目的检测葡萄糖调节蛋白78(GRP78)在胃癌组织中的表达,分析其临床病理学意义,探讨GRP78在胃癌发生发展中的作用。方法收集48例不同临床分期及不同分化程度胃腺癌及28例癌旁正常胃黏膜组织标本,分别应用RT-PCR、Western Blotting和免疫组化检测GRP78 mRNA和蛋白质的表达。结果与正常胃黏膜组织相比,胃癌组织中GRP78 mRNA及蛋白表达水平显著增加(P<0.05)。免疫组化检测GRP78阳性表达定位于胞浆,在正常胃粘膜组织中GRP78阳性表达率为10.7%(3/28),高、中分化与低分化胃癌组织阳性表达率分别为59.4%(19/32)和93.8%(15/16);直径≥5 cm的胃癌组织中GRP78阳性表达率(88.9%,16/18)高于直径<5 cm的胃癌组织(60%,18/30),Ⅲ、Ⅳ期(TNM分期)病例胃癌组织中GRP78阳性表达率(90.5%,19/21)高于Ⅰ、Ⅱ期病例(55.6%,15/27);5年内病情复发或者死亡病例胃癌组织中GRP78阳性表达率(82.8%,24/29)高于5年内病情无复发病例(52.6%,10/19);淋巴结转移胃癌组织中GRP78阳性表达率(96.2%,25/26)明显高于非淋巴结转移胃癌组织(40.9%,9/22),各组间差异均具有统计学意义(P<0.05)。结论 GRP78在胃癌组织中高表达,其表达水平与胃癌肿瘤大小、分化程度、淋巴结转移、临床分期及预后密切相关。Objective To explore the role of GRP78 in the development of gastric carcinoma by analysis of the expression of GRP78 in gastric carcinoma tissue. Methods 48 cases of human gastric carcinoma tissue with different clinical period and differentiation degree and 28 cases of normal gastric tissue were selected. The mRNA and protein of GRP78 were detected by RT-PCR,Western blotting and immunohistochemistry,respectively. Results Compared with normal gastric epithelial tissue,the expression of GRP78 at mRNA and protein level in gastric carcinoma tissue increased significantly,which were showed by RT-PCR and Western Blotting（ P 〈0. 05）. GRP78 protein was mainly distributed in cytoplasm showed by immunohistochemistry. The positive rate of GRP78 in normal gastric tissue,well or moderately-differentiated and poorly-differentiated gastric cancer tissue were 10. 7%（ 3 /28）,59. 4%（ 19 /32） and 93. 8%（ 15 /16）,respectively. The positive rate of GRP78 in gastric carcinoma tissue with diameter ≥5 cm was 88. 9%（ 16 /18）,at Ⅲ,Ⅳ period in TNM staging was 90. 5%（ 19/21）,and with recurrence or death less than 5 years was 82. 8%（ 24/29）,which were higher than that with diameter 5 cm（ 60%,18 /30）,atⅠ,Ⅱ period（ 55. 6%,15 /27）,and with relapse-free in five years（ 52. 6%,10 /19）,respectively. The positive rate of GRP78 in gastric carcinoma tissue with lymphatic metastasis was 96. 2%（ 25 /26）,which was much higher than that of without lymphatic metastasis（ 40. 9%,9 /22）. Conclusion The expression of GRP78 in gastric cancer tissue increased,and GRP78 was involved in the development and progression of gastric cancer,which was highly related to tumor size,differentiation,lymph node metastasis,clinical stage and prognosis of gastric cancer.

关 键 词：胃癌 葡萄糖调节蛋白78 分化 淋巴结转移 临床分期 

分 类 号：R735.2[医药卫生—肿瘤]