陕西地区环氧化酶-2基因的多态性、螺杆菌感染与胃癌易感性的关系  

Association of COX-2 genetic polymorphisms and H. pylori infection with susceptibility of gastric cancer in Shaanxi area

作　　者：陶梅[1] 张玲霞[1] 宋瑛[1] 庄坤[1] 张宁霞[1] 张沥[1] 

机构地区：[1]西安市中心医院消化科,西安710003

出　　处：《山西医科大学学报》2015年第1期17-21,共5页Journal of Shanxi Medical University

基　　金：陕西省科技攻关基金资助项目(2008k09-03)

摘　　要：目的探讨陕西人群环氧化酶-2(COX-2)-765G>C和-1195G>A基因单核苷酸多态性在胃癌患者及健康人群中的分布,探讨其与幽门螺杆菌(Hp)感染的相互作用对GC易感性的影响。方法用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测136例胃癌患者(病例组)和121例体检健康者(对照组)COX-2-765G>C和-1195 G>A位点的基因多态性,ELISA检测两组研究对象Hp感染情况,分析各基因型分布和Hp感染及与胃癌易感性之间的关系。结果在-765G>C位点,病例组携带三种基因型及等位基因C(基因型GC/CC)的频率显著高于对照组(P均<0.05)。与基因型GG相比,胃癌的易感性增加(GC:OR=1.906;CC:OR=2.900;GC+CC:OR=2.081)。在-1195G>A位点,病例组携带基因型GA和AA或携带等位基因A(基因型GA/AA)频率与对照组相比差异无显著性(P均>0.05)。病例组和对照组Hp阳性率分别为56.5%和43.8%,两组间差异显著(P<0.01)。分层分析显示,Hp感染增加了-765 G>C位点C基因型携带者胃癌的易感性(GC/CC:OR=2.081)。结论 COX-2-765 G>C的基因多态性增加了陕西地区胃癌的易感性,且与Hp感染对胃癌易感性有协同作用;而COX-2-1195 G>A位点的基因多态性可能与胃癌的发病无关。Objective To evaluate the association of cyclooxygenase-2 （COX-2） polymorphisms （ - 765 G 〉 C and - 1195G 〉 A） with susceptibility of gastric cancer, and the interaction between COX-2 polymorphisms and H. pylori infection. Methods A total of 136 gastric cancer patients and 121 healthy controls were enrolled in this study. The genetic polymorphisms of COX-2 was determined by PCR-RFLP, and H. pylori infection was determined by ELISA assay. Results The genol.ype frequencies of-765GC, C/C and GC ＋ CC in patients with gastric cancer were higher than those in cotrols（ P 〈 0.05 ）. The risk of gastric cancer patients with GC, CC genotypes and C allele （ GC, CC, GC ＋ CC ） increased compared with GG （ GC ： OR = 1. 906 ; CC ： OR = 2. 900 ; GC ＋ CC ： OR = 2. 081 ）. There was no significant difference in the frequencies of three genotypes and A allele （ GA, AA, GA ＋ AA） of COX-2 - 1195G 〉 A between gastric cancer patients and the controls（ P 〉 0.05 ）. The H. pylori infection rate was significantly higher in patients with gastric cancer than that in controls（56.5% vs 48.3% ,P 〈 0.01 ）. Stratification analysis showed that the allele C carriers of COX-2 gene - 765 G 〉 C（ GC, CC, GC ＋ CC） with H. pylori infection had a higher risk for gastric canc：er（ OR = 2. 471, 95% CI 1. 076 - 5. 675 ）. Conclusion The COX-2 - 765 G 〉 C polymorphism is significantly associated with the susceptibility of gastric cancer,H, pylori infec- tion and COX-2 -765G 〉 C may play a synergic role in gastric cancer pathogenesis. The COX-2 - 1195G 〉 A polymorphism may be not associated with the incidence of gastric cancer.

关 键 词：胃癌 环氧化酶-2(COX-2) 基因多态性 单核苷酸 幽门螺杆菌 

分 类 号：R735.2[医药卫生—肿瘤]